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MRP4/ABCC4 Antibody, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB100-1471

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NB100-1471

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for MRP4/ABCC4 Antibody

Immunogen

Peptide with sequence CNGQPSTLTIFETAL corresponding to C-Terminus according to NP_005836.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28785115). Rat reactivity reported in scientific literature (PMID: 22419695).

Localization

Cytoplasm

Specificity

This antibody is expected to recognize isoform 1 (NP_005836.2) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MRP4/ABCC4 Antibody

Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471] - Staining Human Prostate tissue. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for MRP4/ABCC4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

4 - 6 ug/mL

Immunohistochemistry-Paraffin

4 - 6 ug/mL

Peptide ELISA

Detection limit 1:1000

Western Blot

1-3 ug/ml
Application Notes
ICC/IF successful usage for human primary hepatocytes is reported in scientific literature (PMID: 20395535). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 31669256).WB reactivity reported in (PMID: 25263207). Data obtained from a previous batch (different goat). WB: Approx. 170 kDa band observed in rat renal cortex membranes (calculated MW of 149 kDa band according to rat NP_596902.1 and 150 kDa band according to human NP_005836.2) is provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald, Germany. Data obtained from a previous batch (different goat). IHC-P: Human prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 9 publications using NB100-1471 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MRP4/ABCC4

MRP4 may be an organic anion pump relevant to cellular detoxification

Long Name

Multidrug Resistance-associated Protein 4

Alternate Names

ABCC4, MOATB

Entrez Gene IDs

10257 (Human); 170924 (Rat)

Gene Symbol

ABCC4

UniProt

Additional MRP4/ABCC4 Products

Product Documents for MRP4/ABCC4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MRP4/ABCC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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