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Key Product Details

Species Reactivity

Non-species specific

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

mPlum from Discosoma sp.

Specificity

The rabbit polyclonal antibody recognizes a far red fluorescent protein tag mPlum.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for mPlum Antibody - BSA Free

Western Blot: mPlum AntibodyBSA Free [NBP3-11833]

Western Blot: mPlum AntibodyBSA Free [NBP3-11833]

Western Blot: mPlum Antibody [NBP3-11833] - Analysis of mPlum fluorescent protein using rabbit polyclonal antibody PAb (919) on lysates of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 ug/ml of rabbit anti-mPlum polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for mPlum protein at approximately 30 kDa.
Flow Cytometry: mPlum Antibody - BSA Free [NBP3-11833]

Flow Cytometry: mPlum Antibody - BSA Free [NBP3-11833]

Flow Cytometry: mPlum Antibody [NBP3-11833] - Separation of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 ug/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mPlum/YFP cell suspension.
Flow Cytometry: mPlum Antibody - BSA Free [NBP3-11833]

Flow Cytometry: mPlum Antibody - BSA Free [NBP3-11833]

Flow Cytometry: mPlum Antibody [NBP3-11833] - Surface staining pattern of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained using anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 ug/ml, GAR APC).

Applications for mPlum Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: mPlum

The mPlum is a red fluorescent protein with excitation maximum 589 nm and emission maximum 650 nm. It has around 29 kDa., and it is being used as a fluorescent tag in expression systems.

Alternate Names

Fluorescent protein plum, mPlum Fluorescent Protein, Red Fluorescent Protein, RFP

Additional mPlum Products

Product Documents for mPlum Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for mPlum Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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