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Mouse VEGFR1/Flt-1 Antibody

Catalog # MAB471 | R&D Systems, Inc. a Bio-Techne Brand
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MAB471-100
MAB471-500
MAB471-SP

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 141515

Product Summary for Mouse VEGFR1/Flt-1 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VEGFR1
Ser27-Glu759
Accession # P35969

Specificity

Detects mouse VEGFR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human VEGFR1, recombinant mouse (rm) VEGFR2, or rmVEGFR3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse VEGFR1/Flt-1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus and kidney

Western Blot

1 µg/mL
Sample: Recombinant Mouse VEGFR1/Flt‑1 Fc Chimera (Catalog # 471-F1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB471 in the following applications:

Published Applications

Read 8 publications using MAB471 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR1/Flt-1

VEGFR1 (also called Flt-1) is one of the five receptor tyrosine kinases (RTKs) (VEGFR1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and Tie-2/Tek define a class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGFR1/Flt-1, VEGFR2/KDR/Flk-1, VEGFR3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGFR1 mRNA encodes a 1333 amino acid (aa) residue precursor with a 22 aa residue signal peptide. Mature VEGFR1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGFR1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGFR1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

References

  1. He, Y. et al. (1999) Molecular Endocrinology 13:537.
  2. Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Gene Symbol

FLT1

UniProt

Product Documents for Mouse VEGFR1/Flt-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse VEGFR1/Flt-1 Antibody

For research use only

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