Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody

Bio-Techne includes R&D Systems | Catalog # FAB5871P

Discontinued Product
FAB5871P has been discontinued. View all TGF-beta RI/ALK-5 products.

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 141231

View all TGF-beta RI/ALK-5 Antibodies »

Product Summary for Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta RI/ALK-5
Ala21-Glu121
Accession # BAA05023

Specificity

Detect mouse TGF-beta RI/ALK-5 in flow cytometry. Although this clone detects human and mouse TGF-beta RI/ALK-5 in Western blots, positive results have not been observed on human TGF-beta RI/ALK-5 in flow cytometry. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with rrMIS RII, rhTGF‑ beta RII, rhTGF‑ beta RIIb, or rhTGF‑ beta RIII.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody

Detection of TGF-beta RI/ALK-5 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of TGF‑ beta RI/ALK‑5 in Mouse Splenocytes by Flow Cytometry.

NSA Mouse splenocytes were stained with (A) Rat Anti-Mouse TGF-beta RI/ALK-5 PE-conjugated Monoclonal Antibody (Catalog # FAB5871P) or Rat IgG2a isotype control antibody (Catalog # IC006P) and Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: NSA Mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta RI/ALK-5

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor-like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

References

  1. Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
  2. Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.

Long Name

Transforming Growth Factor beta Receptor I

Alternate Names

ALK-5, SKR4, TGF-bRI, TGFbetaRI, TGFBR1

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Gene Symbol

TGFBR1

UniProt

BAA05023

Additional TGF-beta RI/ALK-5 Products

Product Documents for Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody

For research use only

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