Mouse Reg1 Antibody

Detection of Mouse Reg1 by Western Blot

Reg family proteins stimulated PSCs activation contributing to fibrosis in chronic pancreatitis. (a) Immunofluorescence analysis of alpha-smooth muscle actin ( alphaSMA, an activated PSCs marker, red) in the pancreas sections of indicated genotypes at 2 weeks, and 4 weeks (n = 3–4 mice). Nuclei were counterstained by DAPI (blue). (b) The percentage of epithelial cells with alphaSMA positive signals. Results represent mean ± SEM (n = 3–4 mice). Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison tests among four groups. **P < 0.01 (c) Cropped images of western blot gels of Amylase, alphaSMA, Desmin, Reg1, Reg2, Reg3b of the pancreas of indicated genotypes at 0.5 day after birth(P0.5), 1w and 4w, p44/42 MAPK (ERK 1/2) and Akt were used as the loading control (n = 3 mice). The samples derived from the same experiment and gels were processed in parallel. Images of the entire gels are presented in Supplementary Fig. 5–7. The right panel showed the respective densitometric quantification analysis of the relative intensity of alphaSMA. Densitometric quantification analysis of other proteins is presented in Supplementary Fig. 4.Results represent mean ± SEM (n = 3 mice). Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison tests among four groups. ns: not significant; #P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37500741), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Reg1 by Western Blot

Reg family proteins stimulated PSCs activation contributing to fibrosis in chronic pancreatitis. (a) Immunofluorescence analysis of alpha-smooth muscle actin ( alphaSMA, an activated PSCs marker, red) in the pancreas sections of indicated genotypes at 2 weeks, and 4 weeks (n = 3–4 mice). Nuclei were counterstained by DAPI (blue). (b) The percentage of epithelial cells with alphaSMA positive signals. Results represent mean ± SEM (n = 3–4 mice). Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison tests among four groups. **P < 0.01 (c) Cropped images of western blot gels of Amylase, alphaSMA, Desmin, Reg1, Reg2, Reg3b of the pancreas of indicated genotypes at 0.5 day after birth(P0.5), 1w and 4w, p44/42 MAPK (ERK 1/2) and Akt were used as the loading control (n = 3 mice). The samples derived from the same experiment and gels were processed in parallel. Images of the entire gels are presented in Supplementary Fig. 5–7. The right panel showed the respective densitometric quantification analysis of the relative intensity of alphaSMA. Densitometric quantification analysis of other proteins is presented in Supplementary Fig. 4.Results represent mean ± SEM (n = 3 mice). Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison tests among four groups. ns: not significant; #P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37500741), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Reg1 by Western Blot

Reg deficiency led to the remission of chronic pancreatitis. (a) Representative pancreatic tissue section from mice of the indicated genotype at 8 weeks was stained with H&E or Azan (for collagen, dark blue) and Immunofluorescence staining of alpha-smooth muscle actin ( alphaSMA, an activated PSCs marker, red) (n = 5 mice). Scale bars: H&E, 50 μm; Azan, 100 μm (b) Pancreatic mRNA expression of proinflammatory cytokines interleukin 6(Il6), tumor necrosis factor a (Tnfa) and Il1b of indicated genotypes were assessed by qRT-PCR. The expression amounts of each gene were calculated relative to those of ribosomal protein S3 (Rps3) with the fold change to CP model_Reg+/+ mice. Mean ± SEM (n = 5 mice). Statistical analysis was performed by two-tailed unpaired Student t-test between two groups. *P < 0.05 (c) Cropped images of western blot gels of Amylase, alphaSMA and Reg1 of the pancreas of indicated genotypes at 8 weeks and Akt was used as the loading control (n = 5 mice). The samples derived from the same experiment and gels were processed in parallel. Images of the entire gels and respective densitometric quantification analysis of the relative intensity of protein are presented in Supplementary Fig. 4,8. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37500741), licensed under a CC-BY license. Not internally tested by R&D Systems.