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Mouse PDGF R alpha Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9215G

R&D Systems, part of Bio-Techne
Discontinued Product
FAB9215G has been discontinued. View all PDGF R alpha products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 189208

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha
Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala), predicted
Accession # P26618

Specificity

Detects mouse PDGF R alpha in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images

Detection of PDGF Ra antibody in Mouse Bone Marrow antibody by Flow Cytometry.

Detection of PDGF R alpha in Mouse Bone Marrow by Flow Cytometry.

Mouse bone marrow was stained with Rat Anti-Mouse CD45 APC-conjugated Monoclonal Antibody (Catalog # FAB114A) and either (A) Rat Anti-Mouse PDGF Ra Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB9215G) or (B) Rat IgG2AAlexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.
Detection of PDGF Ra antibody in Mouse Compact Bone Tissue antibody by Flow Cytometry.

Detection of PDGF R alpha in Mouse Compact Bone Tissue by Flow Cytometry.

Mouse compact bone tissue was stained with Rat Anti-Mouse CD45 APC-conjugated Monoclonal Antibody (Catalog # FAB114A) and either (A) Rat Anti-Mouse PDGF Ra Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB9215G) or (B) Rat IgG2AAlexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.

Applications

Application
Recommended Usage

Flow Cytometry

0.5 µg/106 cells
Sample: Mouse bone marrow and mouse compact bone tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PDGF R alpha

PDGF R alpha (Platelet-Derived Growth Factor Receptor alpha), also known as CD140a, is a 165-170 kDa member of the PDGFR (or class III) subfamily, Tyrosine Kinase family, Protein Kinase superfamily of molecules. Mature mouse PDGF R alpha is a 1065 amino acid (aa) type I transmembrane glycoprotein that contains five Ig-like domains within a 504 aa extracellular domain. It is expressed on a wide variety of cell types, both fetal and adult. In the fetus, PDGF R alpha is found on NG2+ oligodendroglial precursors, mesenchyme of both the sclerotome and dermatome (but not myotome), mesenchyme of the lung, hepatoblasts, Leydig cells, and neural crest cells that target the branchial arches. In the adult, PDGF R alpha has been found constitutively or inducibly on hepatocytes, vascular and visceral smooth muscle, Leydig cells, retinal astrocytes, fibroblasts, monocytes, hepatic stellate cells, CD44+ adipocyte precursors, endothelial cells, osteoblasts, and Ptch+ Gli+ pericryptal colonic subepithelial fibroblasts. On the cell surface, PDGF R alpha is a monomer. Upon binding to a dimeric ligand, PDGF R alpha either homodimerizes, or heterodimerizes with PDGF R beta. The PDGF R alpha homodimer binds PDGF-AA, -AB, -BB, and -CC, while the alpha beta-heterodimer binds PDGF-AB, -BB, and -CC. Over aa 25-524, mouse PDGF R alpha shares 93% and 85% aa sequence identity with rat and human PDGF R alpha, respectively. Over the same aa sequence, mouse PDGF R alpha shares only 33% aa sequence identity with mouse PDGF R beta.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Entrez Gene IDs

5156 (Human); 18595 (Mouse)

Gene Symbol

PDGFRA

UniProt

Additional PDGF R alpha Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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