Detects Lymphotoxin betaR/TNFRSF3 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) LT beta R is observed and less than 1% cross-reactivity with recombinant mouse (rm) 4-1BB, rmCD27, rmCD30, rmCD40, rmEDAR, rmFas, rmGITR, rmOPG, rmRANK, rmTNF RI, and rmTNF RII is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Lymphotoxin betaR/TNFRSF3 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse whole blood

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Lymphotoxin betaR/TNFRSF3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta receptor (LTbR), also known as TNFRSF3, TNF RIII, and TNF Rrp, is a transmembrane receptor for the homotrimeric LIGHT/TNFSF14 and the heterotrimeric LTalpha1/beta2. LTbR engagement induces production of IL-7, RANK, TRANCE/RANK L, VEGF-C, the adhesion molecules VCAM-1, ICAM-1, and MAdCAM, and the chemokines CXCL13, CCL19 and CCL21. LTbR is expressed on mesenchymal stromal organizing cells that give rise to stroma of primary (thymus), secondary (tonsils, lymph nodes, and Peyers patches) and tertiary (ectopic inflammatory) lymphoid structures. It is expressed by hepatocytes and is upregulated in regeneration, hepatitis, and hepatocellular carcinoma where it regulates lipid metabolismd. A soluble form of LTbR can be released and functions an inhibitor of transmembrane LTbR.

Long Name

Lymphotoxin beta Receptor

Alternate Names

LTBR, LymphotoxinbR, TNF RIII, TNF Rrp, TNFRSF3

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Gene Symbol

LTBR

UniProt

P50284

Additional Lymphotoxin beta R/TNFRSF3 Products

Product Documents for Mouse Lymphotoxin betaR/TNFRSF3 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Lymphotoxin betaR/TNFRSF3 Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Lymphotoxin betaR/TNFRSF3 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1008

Key Product Details

Species Reactivity

Validated:

Cited:

Applications