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Mouse CD36/SR‑B3 Antibody

Bio-Techne includes R&D Systems | Catalog # AF2519

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AF2519
AF2519-SP

Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse CD36/SR‑B3 Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD36/SR-B3
Gly30-Lys439
Accession # Q3UAI3

Specificity

Detects mouse CD36/SR-B3 in ELISAs and Western blots. In sandwich ELISAs, approximately 20% cross-reactivity with recombinant human CD36 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CD36/SR‑B3 Antibody

Detection of CD36/SR-B3 antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry.

Detection of CD36/SR‑B3 in J774A.1 Mouse Cell Line by Flow Cytometry.

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
CD36/SR-B3 antibody in Mouse Kidney by Immunohistochemistry (IHC-P).

CD36/SR‑B3 in Mouse Kidney.

CD36/SR-B3 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse CD36/SR-B3 by Western Blot

Detection of Mouse CD36/SR-B3 by Western Blot

Liver GADD45 beta controls liver fatty acid handling by cytosolic FABP1 retentionA, BMale 12‐weeks‐old wild‐type (WT; C57Bl/6J) or obese/diabetic (db/db; BKS.Cg‐m+/+ Lepr DB/J) mice with (Ad‐G45b OE) or without (Ad‐NC) prior liver‐restricted GADD45 beta over‐expression were fasted and insulin was injected with livers harvested shortly thereafter and subsequently liver proteins were subjected to immunoblotting for insulin signalling proteins including phosphoprotein kinase B (PKB/Akt; A) and glycogen synthase kinase beta (GSK3b; B). Inserts show representative blots (n = 6/group).C, DRepresentative immunoblots (C) and relative abundance quantifications (D; n = 6) of proteins and phosphoproteins including light chain 3 isoform B (LC3B), S6 kinase 1 (S6K1), p42/44 mitogen activated protein kinase (ERK1/2), eukaryotic initiation factor 2 alpha (eIF2a), glucose regulated protein 78 (GRP78/HSPA5), fatty acid transport protein 2 (FATP2/SLC27A2), cluster determinant 36 (CD36/FAT) and fatty acid binding protein 1 (FABP1) from fasted GADD45 beta+/+ (WT) or GADD45 beta−/− (KO) mice.ERepresentative immunoblots of HNF4a (nuclear marker), BCKDE1A (mitochondrial marker), NTCP (microsomal marker) and ARG1 (cytosolic marker) from liver whole tissue lysate (W) as well as fractionated organelles/intraceuular structures including nuclei (N), mitochondria (MT), microsomes (MS) and cytoplasm (C), from GADD45 beta+/+ (WT) and GADD45 beta−/− (KO) mice.Data information: Data are mean ± SEM. The statistical test used and respective P‐value outputs can be found in Appendix Table S1. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27137487), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CD36/SR‑B3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: J774A.1 mouse monocyte/macrophage cell line

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse kidney

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD36/SR-B3 Fc Chimera (Catalog # 2519-CD)

Mouse CD36/SR-B3 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CD36/SR‑B3 Biotinylated Antibody (Catalog # BAF2519)
  • Standard: Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF (Catalog # 2519-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF2519 in the following applications:

Published Applications

Read 45 publications using AF2519 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD36/SR-B3

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 is 94%, 92%, 84%, and 84% aa identical to the extracellular loops of rat, hamster, human, and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells, and hematopoietic cells such as red blood cells, platelets, and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

References

  1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:795. 
  2. Silverstein, R.L. and M. Febbraio (2000) Curr. Opin. Lipid. 11:483. 
  3. Gruarin, P. et al. (2000) Biochem. Biophys. Res. Commun. 275:446. 
  4. Endemann, G. et al. (1993) J. Biol. Chem. 268:11811. 
  5. Malaud, E. et al. (2002) Biochem. J. 364:507.
  6. Tao, N. et al. (1996) J. Biol. Chem. 271:22315.
  7. Daviet, L. et al. (1997) Thromb. Haemost. 78:897.
  8. Simantov, R. and R.L. Silverstein (2003) Front. Biosci. 8:s874.

Long Name

Scavenger Receptor Class B, Member 3

Alternate Names

CD36, Collagen R, FAT, GPIIIb, GPIV, SCARB3, SR-B3, SRB3, Thrombospondin R

Entrez Gene IDs

948 (Human); 12491 (Mouse)

Gene Symbol

CD36

UniProt

Additional CD36/SR-B3 Products

Product Documents for Mouse CD36/SR‑B3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD36/SR‑B3 Antibody

For research use only

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