Mouse CD36/SR-B3 Antibody

Name: Mouse CD36/SR-B3 Antibody
Brand: R&D Systems
SKU: AF2519

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2519

Citations (27)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Chinese hamster ovary cell line CHO-derived recombinant mouse CD36/SR-B3 Gly30-Lys439 Accession # Q3UAI3
Applications:	CyTOF-ready, ELISA Capture, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse CD36/SR-B3 in ELISAs and Western blots. In sandwich ELISAs, approximately 20% cross-reactivity with recombinant human CD36 is observed.

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Applications for Mouse CD36/SR-B3 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CD36/SR-B3 Fc Chimera (Catalog # 2519-CD)
Flow Cytometry	2.5 µg/10⁶ cells	See below
Immunohistochemistry	3-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Mouse CD36/SR-B3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Mouse CD36/SR‑B3 Biotinylated Antibody (Catalog # BAF2519)

Standard: Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF (Catalog # 2519-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse CD36/SR-B3 Antibody

	Flow Cytometry Detection of CD36/SR‑B3 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
	Immunohistochemistry CD36/SR‑B3 in Mouse Kidney. CD36/SR-B3 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse CD36/SR-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD36/SR-B3

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 is 94%, 92%, 84%, and 84% aa identical to the extracellular loops of rat, hamster, human, and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells, and hematopoietic cells such as red blood cells, platelets, and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

Item	Value
References	Febbraio, M. et al. (2001) J. Clin. Invest. 108:795. Silverstein, R.L. and M. Febbraio (2000) Curr. Opin. Lipid. 11:483. Gruarin, P. et al. (2000) Biochem. Biophys. Res. Commun. 275:446. Endemann, G. et al. (1993) J. Biol. Chem. 268:11811. Malaud, E. et al. (2002) Biochem. J. 364:507. Tao, N. et al. (1996) J. Biol. Chem. 271:22315. Daviet, L. et al. (1997) Thromb. Haemost. 78:897. Simantov, R. and R.L. Silverstein (2003) Front. Biosci. 8:s874.
Long Name	Scavenger Receptor Class B, Member 3
Entrez Gene IDs	948 (Human); 12491 (Mouse)
Alternate Names	BDPLT10, CD36, CD36 antigen, CD36 antigen (collagen type I receptor, thrombospondin receptor), CD36 molecule (thrombospondin receptor), CD_antigen: CD36, CHDS7, Collagen R, FAT, Fatty acid translocase, GP3B, GP4, GPIIIb, GPIV, Glycoprotein IIIb, PAS IV, PAS-4, PAS-4 protein, PASIV, Platelet collagen receptor, SCARB3, SR-B3, SRB3, Thrombospondin R, Thrombospondin receptor, cluster determinant 36, platelet glycoprotein 4, scavenger receptor class B, member 3

Citations for Mouse CD36/SR-B3 Antibody (27)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CD36/SR-B3 Antibody include:

Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot

R Li et al. (2022), Semaglutide May Alleviate Hepatic Steatosis in T2DM Combined with NFALD Mice via miR-5120/ABHD6 Drug Design, Development and Therapy, 16(0):3557-3572.
PMID: 36238196
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

M Dudau et al. (2021), Sea-Buckthorn Seed Oil Induces Proliferation of both Normal and Dysplastic Keratinocytes in Basal Conditions and under UVA Irradiation Journal of personalized medicine, 11(4).
PMID: 33917064
Species: Mouse
Sample Types: Plasma
Applications: Flow Cytometry

RS Gaspar et al. (2021), Maternal and offspring high-fat diet leads to platelet hyperactivation in male mice offspring Scientific Reports, 11(1):1473.
PMID: 33446734
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation

C He et al. (2021), CD36 and LC3B initiated autophagy in B cells regulates the humoral immune response Autophagy, 0(0).
PMID: 33535890
Species: Mouse
Sample Types: Cell Culture Lysates
Applications: Western Blot

M Koga et al. (2020), Hesperidin blocks varenicline-aggravated atherosclerotic plaque formation in apolipoprotein E knockout mice by downregulating net uptake of oxidized low-density lipoprotein in macrophages J. Pharmacol. Sci., 0(0).
PMID: 32173266
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

S Tsuzuki et al. (2018), A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity Biomed. Res., 39(3):117-129.
PMID: 29899187
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

J Yang et al. (2018), Inhibition of CD36 reduces visceral fat accumulation and improves insulin resistance in diet-induced obese BDNF Val66Met mice J. Biol. Chem., 0(0).
PMID: 29914985
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

S Tsuzuki et al. (2018), A role for scavenger receptor B1 as a captor of specific fatty acids in taste buds of circumvallate papillae Biomed. Res., 39(6):295-300.
PMID: 30531159
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

SX Adamson et al. (2018), Metabolomic insights of macrophage responses to graphene nanoplatelets: Role of scavenger receptor CD36 PLoS ONE, 13(11):e0207042.
PMID: 30403754
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

L Håversen et al. (2018), Vimentin deficiency in macrophages induces increased oxidative stress and vascular inflammation but attenuates atherosclerosis in mice Sci Rep, 8(1):16973.
PMID: 30451917
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Q Liu et al. (2018), IL-34 promotes foam cell formation by enhancing CD36 expression through p38 MAPK pathway Sci Rep, 8(1):17347.
PMID: 30478377
Species: Human
Sample Types: Protein
Applications: Western Blot

C Jahansouz et al. (2018), Partitioning of adipose lipid metabolism by altered expression and function of PPAR isoforms after bariatric surgery Int J Obes (Lond), 42(2):139-146.
PMID: 28894292
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

JP Otis et al. (2017), 2Intestinal epithelial cell Caveolin 1 regulates fatty acid and lipoprotein cholesterol plasma levels Dis Model Mech, 0(0).
PMID: 28130355
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

H Shi et al. (2017), CCN3 Regulates Macrophage Foam Cell Formation and Atherosclerosis Am. J. Pathol., 187(6):1230-1237.
PMID: 28527710
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

MCC Manio et al. (2017), CD36 is essential for endurance improvement, changes inï¿½whole-body metabolism, and efficient PPAR-related transcriptional responses in the muscle with exercise training Physiol Rep, 5(10).
PMID: 28526781
Species: Mouse
Sample Types: Recombinant Protein
Applications: Binding Assay

R Biedro? et al. (2016), CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide PLoS ONE, 11(4):e0153558.
PMID: 27073833
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

(2016), Cell-surface CD36 in monocyte/macrophage contributes to phagocytosis during the resolution phase of ischemic stroke in mice J Biol Chem, 0(0).
PMID: 27646002
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Samovski D et al. (2015), Regulation of AMPK activation by CD36 links fatty acid uptake to beta-oxidation. Diabetes, 64(2):353-9.
PMID: 25157091
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Lee S et al. (2015), Expression of CD36 by Olfactory Receptor Cells and Its Abundance on the Epithelial Surface in Mice. PLoS ONE, 10(7):e0133412.
PMID: 26186589
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Pietka et al. (2012), CD36 protein influences myocardial Ca2+ homeostasis and phospholipid metabolism: conduction anomalies in CD36-deficient mice during fasting. J Biol Chem, 287(46):38901-12.
PMID: 23019328
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Nagashima M et al. (2011), Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice. Diabetologia, 54(10):2649-59.
PMID: 21786155
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Pi J et al. (2010), Deficiency in the nuclear factor E2-related factor-2 transcription factor results in impaired adipogenesis and protects against diet-induced obesity. J. Biol. Chem., 285(12):9292-300.
PMID: 20089859
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Makinen PI et al. (2010), Silencing of either SR-A or CD36 reduces atherosclerosis in hyperlipidaemic mice and reveals reciprocal upregulation of these receptors. Cardiovasc. Res., 88(3):530-8.
PMID: 20634212
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Okutsu R et al. (2009), Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages. Atherosclerosis, 204(2):405-11.
PMID: 19108834
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Kim E et al. (2008), CD36/fatty acid translocase, an inflammatory mediator, is involved in hyperlipidemia-induced exacerbation in ischemic brain injury. J. Neurosci., 28(18):4661-70.
PMID: 18448643
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Qiao L et al. (2008), Transcriptional regulation of fatty acid translocase/CD36 expression by CCAAT/enhancer-binding protein alpha. J. Biol. Chem., 283(14):8788-95.
PMID: 18263877
Species: Rat
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Chang Y et al. (2007), Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis. J. Cell. Sci., 120(0):3053-63.
PMID: 17684062

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