Mouse CD14 Alexa Fluor® 647-conjugated Antibody

Name: Mouse CD14 Alexa Fluor® 647-conjugated Antibody
Brand: R&D Systems
SKU: FAB982R

R&D Systems, part of Bio-Techne | Catalog # FAB982R

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Product Datasheet / COA / SDS

Discontinued Product

FAB982R has been discontinued. View all CD14 products.

Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse CD14 Ala18-Pro345 (predicted) Accession # P10810
Applications:	Flow Cytometry
Label:	Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)
Specificity:	Detects mouse CD14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD14 is observed.

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Technical Data
Citations / Reviews

Applications for Mouse CD14 Alexa Fluor® 647-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25-1 µg/10⁶ cells	Mouse whole blood

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The mouse CD14 cDNA encodes a 366 amino acid (aa) residue precursor protein with a 15 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Mouse CD14 has five potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of mouse CD14 is approximately 65% and 82% identical to the human and rat proteins, respectively. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

Item	Value
References	Wright, S.D. et al. (1990) Science 249:1431. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744. Beutler, B. (2000) Current Opinion in Immunology 12:20. Stelter, F. (2000) Chem. Immunol. 74:25.
Entrez Gene IDs	929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
Alternate Names	CD14, CD14 antigen, CD14 molecule, Myeloid cell-specific leucine-rich glycoprotein, monocyte differentiation antigen CD14

Product Specific Notices for Mouse CD14 Alexa Fluor® 647-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Mouse CD14 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB982R

Key Product Details

Applications for Mouse CD14 Alexa Fluor® 647-conjugated Antibody

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: CD14

Product Specific Notices for Mouse CD14 Alexa Fluor® 647-conjugated Antibody

Customer Reviews for Mouse CD14 Alexa Fluor® 647-conjugated Antibody

Product Documents for Mouse CD14 Alexa Fluor® 647-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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