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Mouse ASGR1/ASGPR1 Antibody

Catalog # AF2755 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Size / Price

Key Product Details

Species Reactivity



Blockade of Receptor-ligand Interaction, Western Blot



Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse ASGR1/ASGPR1 Antibody


Mouse myeloma cell line NS0-derived recombinant mouse ASGPR1
Accession # Q91Y84


Detects mouse ASGPR1 in direct ELISAs and Western blots.







Scientific Data Images for Mouse ASGR1/ASGPR1 Antibody

Detection of Mouse ASGR1/ASGPR1 antibody by Western Blot.

Detection of Mouse ASGR1/ASGPR1 by Western Blot.

Western blot shows lysate of mouse liver tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ASGR1/ASGPR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2755) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASGR1/ASGPR1 at approximately 40-46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse ASGR1/ASGPR1 Antibody

Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1.5-5 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated  beta‑Galactosamine-N-Acetyl-Polyacrylamide to immobilized Recombinant Mouse ASGPR1 (Catalog # 2755-AS) coated at 2.5 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.

Western Blot

0.25 µg/mL
Sample: Mouse liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.


Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASGR1/ASGPR1

The mouse asialoglycoprotein receptor (ASGP-R) is an endocytic recycling receptor that belongs to the long-form subfamily of the C-type/Ca++-dependent lectin family (1-3). It is a complex of two noncovalently-linked subunits, a major 42 kDa glycoprotein (ASGPR1) and a minor 51 kDa glycoprotein (ASGR2). The major mouse ASGP-R subunit, ASGPR1, is synthesized as a 284 amino acid (aa) type II transmembrane (TM) protein that contains a 39 aa cytoplasmic region, a 21 aa TM segment, and a 224 aa extracellular domain (ECD) (4‑6). The ECD contains two important structural regions. The first is a stalk region of 56 aa (aa’s # 59-117) that contributes to noncovalent oligomerization. The second is a 118 aa, carbohydrate-binding, Ca++-dependent C-type lectin domain (aa’s 160-277) that is unusually stabilized by three Ca++ ions (3, 5). There are two potential alternate splice forms for ASGPR1. Both are TM and show a deletion of the C-type lectin domain. One is 113 aa in length and shows a deletion of aa’s # 114-284 (7). The second is 132 aa in length and shows a deletion of aa’s 118-146 and aa’s 162 - 284 (8). Mouse ASGPR1 ECD is 89% and 79% aa identical to the ASGPR1 ECD in rat and human, respectively. The minor mouse ASGP-R subunit, ASGR2, is also a C-type lectin that shares the same structural organization as ASGR-1. It is 301 aa in length and has two 45 kDa and 51 kDa differentially-glycosylated isoforms (4, 6, 9). The ECD of ASGR2 is 50% aa identical to the ECD of ASGPR1. Although ASGPR1 and 2 can be expressed individually, a fully functional and stable ASGP-R requires simultaneous expression of both subunits (10-12). The stoichiometry of a functional ASGP-R is suggested to be either a 2:2, 3:1 or 3:2 ratio of ASGPR1:ASGR2 (13, 14). ASGPR1 is reported to bind Gal (non-reducing), GalNAc, and sialic acid alpha2,6GalNAc (3, 15, 16). This is generally in the context of triantennary or tetraantennary configurations (2).


  1. Stockert, R. J. (1995) Physiol. Rev. 75:591.
  2. Weigel, P.H. and J.H.N. Yik (2002) Biochim. Biophys. Acta 1572:341.
  3. Meier, M. et al. (2000) J. Mol. Biol. 300:857.
  4. Takezawa, R. et al. (1993) Biochim. Biophys. Acta 1172 :220.
  5. Monroe, R.S. and B.E. Huber (1994) Gene 148:237.
  6. Sanford, J.P. et al. (1988) DNA 7:721.
  7. Heath, P. (2005) GenBank Accession # Q5NCV2.
  8. Heath, P. (2005) GenBank Accession # Q5NCV1.
  9. Sanford, J.P. and D. Doyle (1990) Biochim. Biophys. Acta 1087:259.
  10. Braun, J.R. et al. (1996) J. Biol. Chem. 271:21160.
  11. Ishibashi, S. et al. (1994) J. Biol. Chem. 269:27803.
  12. Tozawa, R. et al. (2001) J. Biol. Chem. 276:12624.
  13. Bider, M.D. et al. (1996) J. Biol. Chem. 271:31996.
  14. Lodish, H. (1991) Trends Biochem. Sci. 16:374.
  15. Westerlind, U. et al. (2004) Glyconj. J. 21:227.
  16. Park, E.I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17125.

Long Name

Asialoglycoprotein Receptor 1

Alternate Names


Entrez Gene IDs

432 (Human); 11889 (Mouse); 24210 (Rat)

Gene Symbol



Product Documents for Mouse ASGR1/ASGPR1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse ASGR1/ASGPR1 Antibody

For research use only

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