Key Product Details
Product Summary for Mouse ASGR1/ASGPR1 Antibody
Accession # NP_033844
Scientific Data Images for Mouse ASGR1/ASGPR1 Antibody
Mouse ASGR1/ASGPR1 ELISA Standard Curve.Recombinant Mouse ASGR1/ASGPR1 protein was serially diluted 2-fold and captured by Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27552) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Applications for Mouse ASGR1/ASGPR1 Antibody
This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27552).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The mouse asialoglycoprotein receptor (ASGP-R) is an endocytic recycling receptor that belongs to the long-form subfamily of the C-type/Ca++-dependent lectin family (1‑3). It is a complex of two non‑covalently linked subunits, a major 42 kDa glycoprotein (ASGPR1), and a minor 51 kDa glycoprotein (ASGR2). The major mouse ASGP‑R subunit, ASGPR1, is synthesized as a 284 amino acid (aa) type II transmembrane (TM) protein that contains a 39 aa cytoplasmic region, a 21 aa TM segment, and a 224 aa extracellular domain (ECD) (4-6). The ECD contains two important structural regions. The first is a stalk region of 56 aa (aa’s 59-117) that contributes to non-covalent oligomerization. The second is a 118 aa, carbohydrate-binding, Ca++-dependent C-type lectin domain (aa’s 160-277) that is unusually stabilized by three Ca++ ions (3, 5). There are two potential alternate splice forms for ASGPR1. Both are TM and show a deletion of the C-type lectin domain. One is 113 aa in length and shows a deletion of aa’s 114-284 (7). The second is 132 aa in length and shows a deletion of aa’s 118-146 and aa’s 162-284 (8). Mouse ASGPR1 ECD is 89% and 79% aa identical to the ASGPR1 ECD in rat and human, respectively. The minor mouse ASGP-R subunit, ASGR2, is also a C-type lectin that shares the same structural organization as ASGR-1. It is 301 aa in length and has two 45 kDa and 51 kDa differentially-glycosylated isoforms (4, 6, 9). The ECD of ASGR2 is 50% aa identical to the ECD of ASGPR1. Although ASGPR1 and 2 can be expressed individually, a fully functional and stable ASGP-R requires simultaneous expression of both subunits (10-12). The stoichiometry of a functional ASGP-R is suggested to be either a 2:2, 3:1 or 3:2 ratio of ASGPR1:ASGR2 (13, 14). ASGPR1 is reported to bind Gal (nonreducing), GalNAc, and sialic acid alpha2,6GalNAc (3, 15, 16). This is generally in the context of triantennary or tetraantennary configurations (2).
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Product Specific Notices for Mouse ASGR1/ASGPR1 Antibody
For research use only