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MOG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48458

Novus Biologicals, part of Bio-Techne
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NBP3-48458

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human Myelin. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit MOG Antibody (NBP3-48458) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MOG Antibody

MOG Antibody

Western Blot: MOG Antibody [NBP3-48458] -

Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MOG antibody [C2C3], C-term (NBP3-48458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MOG Antibody

Immunocytochemistry/ Immunofluorescence: MOG Antibody [NBP3-48458] -

MOG antibody [C2C3], C-term detects MOG protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MOG protein stained by MOG antibody [C2C3], C-term (NBP3-48458) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] diluted at 1:500.
Blue: Fluoroshield with DAPI .
MOG Antibody

Immunohistochemistry-Frozen: MOG Antibody [NBP3-48458] -

MOG antibody [C2C3], C-term detects MOG protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MOG protein stained by MOG antibody [C2C3], C-term (NBP3-48458) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.
Blue: Fluoroshield with DAPI .

Applications for MOG Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MOG

Myelin is produced by oligodendrocytes in the CNS and Schwann cells in the peripheral nervous system (PNS). Defining the differences between CNS and PNS myelin may provide insights into the process of myelination and the pathogenesis of certain idiopathic diseases involving myelin such as multiple sclerosis, certain leukodystrophies, and other myelinopathies. Myelin is composed of about 80% lipid fat and about 20% protein. Some of the proteins that make up myelin are Myelin basic protein (MBP), Myelin oligodendrocyte glycoprotein (MOG) and Proteolipid protein (PLP). Myelin is made up primarily of a glycolipid called galactocerebroside. The intertwining of the hydrocarbon chains of sphingomyelin serve to strengthen the myelin sheath.

Long Name

Myelin Oligodendrocyte Glycoprotein

Alternate Names

MGC26137, MOG Ig-AluB, MOGIG2, myelin oligodendrocyte glycoprotein, myelin-oligodendrocyte glycoprotein

Additional MOG Products

Product Documents for MOG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MOG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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