MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB200-114

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8B4

Format

BSA Free

Concentration

1 mg/ml

View all MMP-2 Antibodies »

Product Specifications

Immunogen

Activated recombinant human MMP-2.

Specificity

This is specific for pro and active MMP2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free

Western Blot: MMP-2 Antibody (8B4)(Pro and Active)BSA Free [NB200-114]

Western Blot: MMP-2 Antibody (8B4)(Pro and Active)BSA Free [NB200-114]

Western Blot: MMP-2 Antibody (8B4) - (Pro and Active) [NB200-114] - Analysis of MMP2 expression in ProMMP2 (1), Active MMP2 (2) and human pressure ulcer biopsy (3).
Immunohistochemistry-Paraffin: MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free [NB200-114]

Immunohistochemistry-Paraffin: MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free [NB200-114]

Immunohistochemistry-Paraffin: MMP-2 Antibody (8B4) - (Pro and Active) [NB200-114] - Detection of MMP-2 staining in FFPE human ovary tissue. Primary antibody was used at 2.5 ug/ml with the avidin-biotin-peroxidase detection method.
MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free

Western Blot: MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free [NB200-114] -

TBX3 promotes 3D spheroid invasion. (A) 3D spheroid invasion assay of EV and TBX3 hMSCs. Representative images of the 3D invading spheroids at 0, 24 and 48 h. (40X; EVOS M5000 Imaging System; scale bars = 600 um). (B) Quantification of the invasive area. Student’s t-test was used to compare between groups, ***p < 0.001; error bars represent mean +/- SEM (n=3). (C) Western blot analysis of MMP2 and MMP9 expression in 3D hMSC spheroids (n=2). For western blotting beta-actin was used as a loading control. Densitometry readings were obtained using Fiji and protein expression levels are represented as a ratio of protein of interest/ beta-actin normalized to TBX3 #1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35145908), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free

Application
Recommended Usage

ELISA

0.03 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Frozen

2ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Knockdown Validated

reported in scientific literature (PMID 31439546)

Simple Western

1:50

Western Blot

1-2ug/ml
Application Notes
In Western blot, a band is seen at 70 and 62kDa representing the MMP2 band-pair. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MMP-2

MMP2 is a member of the Matrix Metalloproteinase (MMP) family, which are involved in extracellular matrix degradation under both normal physiological and disease processes. Specifically, MMP-2 is responsible for degrading collagens type IV, V, VII, and X and gelatin type I. Other reported functions of MMP 2 include angiogenesis, tumor invasion, tissue repair, remodeling of the vasculature, inflammation, and atherosclerotic plaque rupture. MMP-2 secretion is activated by the Ras signaling pathway. The MMP2 antibody clone MMP2/8B4 was created against activated recombinant human MMP2, and will specifcally detect both the active and inactive forms. This MMP-2 antibody has been thoroughly characterized in most common immunological applications and has been cited in a number of published journal articles.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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