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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for MKRN1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 432 and the C-terminus (residue 482) of human makorin, ring finger protein, 1 using the numbering given in entry NP_038474.1 (GeneID 23608).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MKRN1 Antibody

Western Blot: MKRN1 Antibody [NB100-55304] - Detection of human and mouse MKRN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MKRN1 antibody NB100-55304 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry: MKRN1 Antibody [NB100-55304] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- MKRN1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: MKRN1 Antibody [NB100-55304] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at for WB at 0.1 ug/ml.

Applications for MKRN1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using NB100-55304 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MKRN1

MKRN1 was identified as a repressor of c-Jun, androgen receptor, and retinoic acid receptor transcriptional activity. MKRN1 is a RING zinc finger protein that has been demonstrated to function as an E3 ubiquitin ligase. The repressive activity of MKRN1 is not dependent on its ubiquitin ligase activity. MKRN1 also appears to function as a transactivator of RNA polymerase II dependent transcription.

Alternate Names

E3 ubiquitin-protein ligase makorin-1, EC 6.3.2.-, FLJ21334, makorin ring finger protein 1, RNF61RING finger protein 61

Entrez Gene IDs

23608 (Human)

Gene Symbol

MKRN1

Product Documents for MKRN1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MKRN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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