MKRN1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55304
![Western Blot: MKRN1 Antibody [NB100-55304]](https://resources.bio-techne.com/images/products/MKRN1-Antibody-Western-Blot-NB100-55304-img0003.jpg "Western Blot: MKRN1 Antibody [NB100-55304]")
![Immunohistochemistry-Paraffin: MKRN1 Antibody [NB100-55304]](https://resources.bio-techne.com/images/products/MKRN1-Antibody-Immunohistochemistry-Paraffin-NB100-55304-img0001.jpg "Immunohistochemistry-Paraffin: MKRN1 Antibody [NB100-55304]")
![Immunoprecipitation: MKRN1 Antibody [NB100-55304]](https://resources.bio-techne.com/images/products/MKRN1-Antibody-Immunoprecipitation-NB100-55304-img0006.jpg "Immunoprecipitation: MKRN1 Antibody [NB100-55304]")
![Western Blot: MKRN1 Antibody [NB100-55304]](https://resources.bio-techne.com/images/products/MKRN1-Antibody-Western-Blot-NB100-55304-img0007.jpg "Western Blot: MKRN1 Antibody [NB100-55304]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 432 and the C-terminus (residue 482) of human makorin, ring finger protein, 1 using the numbering given in entry NP_038474.1 (GeneID 23608).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MKRN1 Antibody - BSA Free (NB100-55304) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-MKRN1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MKRN1 Antibody - BSA Free
Western Blot: MKRN1 Antibody [NB100-55304]
Western Blot: MKRN1 Antibody [NB100-55304] - Detection of human and mouse MKRN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MKRN1 antibody NB100-55304 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Immunohistochemistry: MKRN1 Antibody [NB100-55304]](https://resources.bio-techne.com/images/products/MKRN1-Antibody-Immunohistochemistry-NB100-55304-img0005.jpg "Immunohistochemistry: MKRN1 Antibody [NB100-55304]")
Immunohistochemistry: MKRN1 Antibody [NB100-55304]
Immunohistochemistry: MKRN1 Antibody [NB100-55304] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- MKRN1 used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for MKRN1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MKRN1
Alternate Names
E3 ubiquitin-protein ligase makorin-1, EC 6.3.2.-, FLJ21334, makorin ring finger protein 1, RNF61RING finger protein 61
Entrez Gene IDs
23608 (Human)
Gene Symbol
MKRN1
UniProt
Additional MKRN1 Products
Product Documents for MKRN1 Antibody - BSA Free
Product Specific Notices for MKRN1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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