Methylglyoxal Antibody (9E7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59368

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Non-species specific

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 9E7

Format

BSA Free

Concentration

1 mg/ml

View all Methylglyoxal Antibodies »

Product Specifications

Immunogen

Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Methylglyoxal Antibody (9E7) - BSA Free

Western Blot: Methylglyoxal Antibody (9E7) [NBP2-59368]

Western Blot: Methylglyoxal Antibody (9E7) [NBP2-59368]

Western Blot: Methylglyoxal Antibody (9E7) [NBP2-59368] - Western Blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7 (NBP2-59368). Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (NBP2-59368) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/ Immunofluorescence: Methylglyoxal Antibody (9E7) [NBP2-59368]

Immunocytochemistry/ Immunofluorescence: Methylglyoxal Antibody (9E7) [NBP2-59368]

Immunocytochemistry/Immunofluorescence: Methylglyoxal Antibody (9E7) [NBP2-59368] - Tissue: Humnan embryonic kidney cells (HEK293). Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (SMC-516) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Immunohistochemistry: Methylglyoxal Antibody (9E7) [NBP2-59368]

Immunohistochemistry: Methylglyoxal Antibody (9E7) [NBP2-59368]

Immunohistochemistry: Methylglyoxal Antibody (9E7) [NBP2-59368] - Immunohistochemistry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7 (NBP2-59368). Tissue: Heart. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (NBP2-59368) at 1:25 for 3 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Actin (C) Methylgyloxal Antibody (D) Composite.
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Applications for Methylglyoxal Antibody (9E7) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Methylglyoxal

Methylglyoxal is the organic compound with the formula CH3C(O)CHO. Gaseous methylglyoxal has two carbonyl groups, an aldehyde and a ketone but in the presence of water, it exists as hydrates and oligomers. It is a reduced derivative of pyruvic acid.

Alternate Names

2-oxopropanal, Pyruvaldehyde

Additional Methylglyoxal Products

Product Documents for Methylglyoxal Antibody (9E7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Methylglyoxal Antibody (9E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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