Methyl-Lysine Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78104
Key Product Details
Species Reactivity
Non-species specific
Applications
Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity-purified Methyl-Lysine Antibody was prepared from whole rabbit serum produced by repeated immunizations with Methyl-Lysine conjugated KLH.
Reactivity Notes
The cross reactivity with tri-methyl lysine has not been tested. There are no cross reactions with acetylated proteins
Specificity
Reactivity is specific for Methylated Lysine residues (mono, di).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using methylated lysine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Applications for Methyl-Lysine Antibody - BSA Free
Application
Recommended Usage
ELISA
1:1000-1:20000
Western Blot
1:500-1:3000
Application Notes
This affinity-purified antibody is suitable for use in ELISA, immunohistochemistry, western blotting and immunoprecipitation assays. Although not tested, this antibody is likely functional in RIA, and flow cytometry.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0, 50% (v/v) Glycerol
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: Methyl-Lysine
Alternate Names
Dimethylysine, Methyl lysine, methyllysine, monomethyl lysine, N epsilon dimethyl lysine, N epsilon mono methyllysine, N epsilon trimethyl lysine, N(epsilon) trimethyl lysine, Trimethyl lysine
Additional Methyl-Lysine Products
Product Documents for Methyl-Lysine Antibody - BSA Free
Product Specific Notices for Methyl-Lysine Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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