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MBP Antibody (12)

Catalog # NB600-717 | Novus Biologicals a Bio-Techne Brand
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NB600-717
NB600-717SS

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Feline, Guinea Pig, Mammal, Rabbit, Sheep

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Radioimmunodiffusion, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 12

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Summary for MBP Antibody (12)

Immunogen

Bovine MBP

Epitope

Amino acids 82-87.

Reactivity Notes

Canine reactivity reported in scientific literature (PMID:32773406). Feline reactivity reported in scientific literature (PMID:33091431).

Localization

Cytoplasmic side of myelin

Specificity

NB600-717 reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Clone 12 has been reported as being suitable for use in Western blotting.

Marker

Oligodendrocyte Marker, Myelin Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for MBP Antibody (12)

Western Blot: MBP Antibody (12) [NB600-717] - Mouse Brain Tissue lysate probed with Rat anti MBP.
Immunocytochemistry/Immunofluorescence: MBP Antibody (12) [NB600-717] - Histological and mRNA analysis of the inflammatory cytokines in the vehicle- or etifoxine-treated mice at onset of clinical symptoms. At day 10 p.i., drug treated animals showed significant differences in MBP staining. Animals treated with etifoxine showed an increase in retention of percentage of MBP coverage (*p = 0.001). Image collected and cropped by CiteAb from the following publication (http://embomolmed.embopress.org/cgi/doi/10.1002/emmm.201202124), licensed under a CC-BY license.
Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse brain corpus callosum. Image from verified customer review.

Applications for MBP Antibody (12)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using NB600-717 in the following applications:

Published Applications

Read 41 publications using NB600-717 in the following applications:

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

0.1 M Tris

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBP

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Entrez Gene IDs

4155 (Human); 17196 (Mouse); 24547 (Rat); 618684 (Bovine)

Gene Symbol

MBP

UniProt

Product Documents for MBP Antibody (12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MBP Antibody (12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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