Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48138

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse (Negative), Porcine (Negative), Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # D11

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Macrophage and Histiocytoma Marker Antibodies »

Product Specifications

Immunogen

Membrane preparation from human hepatocytes

Reactivity Notes

Does not react with Mouse, Rat or Porcine.

Localization

Cell Surface & Cytoplasmic

Specificity

In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this monoclonal antibody and a standard CD68 monoclonal antibody showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This monoclonal antibody shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This monoclonal antibody does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

Marker

Macrophage & Histiocytoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted [NBP2-48138]

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted [NBP2-48138]

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted [NBP2-48138] - Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).
Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted [NBP2-48138]

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted [NBP2-48138]

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted [NBP2-48138] - Human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).

Applications for Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Molecular weight of antigen: 125kDa -135kDa Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Macrophage and Histiocytoma Marker

Additional Macrophage and Histiocytoma Marker Products

Product Documents for Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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