M13 Bacteriophage Protein g8p Antibody (RL-ph2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09081
Key Product Details
Species Reactivity
Virus
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # RL-ph2
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RL-ph2 is a mouse monoclonal IgG2a, kappa antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a Balb/c mouse immunized with isolated M13 phage coat proteins.
Reactivity Notes
Only reacts with M13 phages and does not react with proteins of any animal species.
Specificity
RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Scientific Data Images for M13 Bacteriophage Protein g8p Antibody (RL-ph2) - BSA Free
Western Blot: M13 Bacteriophage Protein g8p Antibody (RL-ph2) [NBP3-09081]
Western Blot: M13 Bacteriophage Protein g8p Antibody (RL-ph2) [NBP3-09081] - RL-ph2 recognizing M13 phage coat protein g8p at 1:1000 dilution.Applications for M13 Bacteriophage Protein g8p Antibody (RL-ph2) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:25 - 1:200
Western Blot
1:100 - 1:1000
Application Notes
This antibody is particularly useful for immunoblotting of separated phage proteins, and is also suitable for detection of M13 phages in multistep immunoblotting procedures, immunocytochemistry, flow cytometry, affinity chromatography and ELISA..
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: M13 Bacteriophage Protein g8p
UniProt
Additional M13 Bacteriophage Protein g8p Products
Product Documents for M13 Bacteriophage Protein g8p Antibody (RL-ph2) - BSA Free
Product Specific Notices for M13 Bacteriophage Protein g8p Antibody (RL-ph2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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