Western blot shows lysates of Aedes aegypti LTRIN peptide. PVDF membrane was probed with 2 µg/mL of Mouse Anti-LTRIN Monoclonal Antibody (Catalog # MAB10178) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LTRIN at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LTRIN ELISA Standard Curve.

LTRIN protein was serially diluted 2-fold and captured by Mouse Anti-LTRIN Monoclonal Antibody (Catalog # MAB10178) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-LTRIN Monoclonal Antibody (Catalog # MAB101781) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for LTRIN Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-LTRIN Monoclonal Antibody (Catalog # MAB101781).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Western Blot

2 µg/mL
Sample: Aedes aegypti LTRIN peptide

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LTRIN

Salivary gland protein LTRIN, a 15 Kilodalton protein from mosquito Aedes aegypti, has been shown to facilitate the transmission of Zika virus (ZIKV) probably by interfering with signaling through the lymphotoxin-beta receptor (LT betaR).

Long Name

Multiple Coagulation Factor Deficiency Protein 2

Entrez Gene IDs

23687673 (Insect)

Gene Symbol

LOC23687673

Additional LTRIN Products

Product Documents for LTRIN Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for LTRIN Antibody

For research use only

Citations
Reviews

LTRIN Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10178

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all LTRIN Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for LTRIN Antibody

Detection of LTRIN by Western Blot.