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Lgr5/GPR49 Antibody (4H8)

Catalog # NBP3-15237 | Novus Biologicals a Bio-Techne Brand
Recombinant Monoclonal Antibody
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NBP3-15237

Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4H8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Lgr5/GPR49 Antibody (4H8)

Immunogen

A synthesized peptide derived from human LGR5/GPR49. UniProt ID (O75473).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lgr5/GPR49 Antibody (4H8)

Western Blot: Lgr5/GPR49 Antibody (4H8) [NBP3-15237] - Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue. All lanes: Lgr5/GPR49 antibody at 1:1500. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 100, 98, 93 KDa. Observed band size: 100 KDa.
Flow Cytometry: Lgr5/GPR49 Antibody (4H8) [NBP3-15237] - Overlay histogram showing HepG2 cells stained with Lgr5/GPR49 antibody (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Lgr5/GPR49 Antibody (4H8)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Lgr5/GPR49

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Gene Symbol

LGR5

Product Documents for Lgr5/GPR49 Antibody (4H8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lgr5/GPR49 Antibody (4H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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