Lewis A,B Blood Group Antigens Antibody (HEA164) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12056

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # HEA164

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Lewis A,B Blood Group Antigens Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunising mice with human carcinoma cells.

Specificity

This antibody is specific for Lewis A,B Blood Group Antigens carbohydrate antigens.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images

Western Blot: Lewis A,B Blood Group Antigens Antibody (HEA164)ChimericAzide and BSA Free [NBP3-12056]

Western Blot: Lewis A,B Blood Group Antigens Antibody (HEA164)ChimericAzide and BSA Free [NBP3-12056]

Western Blot: Lewis A,B Blood Group Antigens Antibody (HEA164) - Chimeric [NBP3-12056] - Human colorectal carcinoma tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HEA164 (NBP3-12056) at 0.001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Lewis A,B Blood Group Antigens Antibody (HEA164) - Chimeric - Azide and BSA Free [NBP3-12056]

Immunocytochemistry/ Immunofluorescence: Lewis A,B Blood Group Antigens Antibody (HEA164) - Chimeric - Azide and BSA Free [NBP3-12056]

Immunocytochemistry/Immunofluorescence: Lewis A,B Blood Group Antigens Antibody (HEA164) - Chimeric [NBP3-12056] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of HEA164 (NBP3-12056) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12056, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Lewis A,B Blood Group Antigens

Alternate Names

Le-a, Le-b, Lewis A, Lewis A Blood Group Antigen, Lewis B, lewis B blood group antigen

Additional Lewis A,B Blood Group Antigens Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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