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Leukotriene A4 Hydrolase/LTA4H Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90368

Novus Biologicals, part of Bio-Techne
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NBP1-90368

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YLERHICGRLFGEKFRHFNALGGWGELQNSVKTFGETHPFTKLVVDLTDIDPDVAYSSVPYEKGFALLFYLEQLLGGPEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVDWNAWLYSPGLPPIKPNYDMTL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Leukotriene A4 Hydrolase/LTA4H Antibody - BSA Free

Leukotriene A4 Hydrolase/LTA4H Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: Leukotriene A4 Hydrolase/LTA4H Antibody - BSA Free [NBP1-90368]

Immunocytochemistry/ Immunofluorescence: Leukotriene A4 Hydrolase/LTA4H Antibody - BSA Free [NBP1-90368]

Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90368]

Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90368]

Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90368] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90368]

Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90368]

Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90368] - Analysis using Anti-LTA4H antibody NBP1-90368 (A) shows similar pattern to independent antibody NBP1-90375 (B).

Applications for Leukotriene A4 Hydrolase/LTA4H Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Simple Western

1:10

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF FFixation Permeabilization: Use PFA/Triton X-100. Simple Western referenced in scientific literature (PMID: 32026202)
See Simple Western Antibody Database for Simple Western validation: separated by Size

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Leukotriene A4 Hydrolase/LTA4H

Hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). The enzyme also has some peptidase activity

Alternate Names

LTA4H

Gene Symbol

LTA4H

Additional Leukotriene A4 Hydrolase/LTA4H Products

Product Documents for Leukotriene A4 Hydrolase/LTA4H Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Leukotriene A4 Hydrolase/LTA4H Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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