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Laminin Antibody (LAM-89)

Catalog # NB600-883 | Novus Biologicals a Bio-Techne Brand
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NB600-883
NB600-883-0.025ml

Key Product Details

Species Reactivity

Human, Mouse, Porcine, Canine (Negative), Chicken (Negative), Feline, Fish, Goat (Negative), Guinea Pig (Negative), Rabbit (Negative), Rat (Negative), Reptile (Negative), Sheep (Negative)

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LAM-89

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Summary for Laminin Antibody (LAM-89)

Immunogen

Laminin Antibody (LAM-89) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Reactivity Notes

Should not react with carp, lizard, frog, snake. Stains basal membranes of human, pig and cat. Fish reactivity reported in scientific literature (PMID: 26517388). Human and mouse reactivity reported in scientific literature.

Localization

Extracellular. Found in the basement membranes.

Specificity

Laminin Antibody (LAM-89) specificity was determined by immunoperoxidase and immunofluorescent labeling of protease-digested, formalin-fixed, paraffin-embedded human or animal tissue sections, localizing specifically the basal membrane of blood vessels, epithelium, nerve, and muscle tissues. Localizes specifically in the basal membrane of blood vessels, epithelium, nerve and muscle fibers. Shows no cross-reaction with collagen type IV, fibronectin, vitronectin or chondroitin sulfates type A, B and C using a dot blot immunoassay. This antibody may be used for identification of laminin in various human tissues and bodily fluids.

Marker

Basement Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

337 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Laminin Antibody (LAM-89)

Immunohistochemistry: Laminin Antibody (LAM-89) [NB600-883] - Formalin-fixed, paraffin-embedded human tongue section, enzyme-treated was stained with 1:2,000 Monoclonal Anti-Laminin Clone: LAM-89. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunohistochemistry: Laminin Antibody (LAM-89) [NB600-883] - Analysis in human placenta section with Laminin Antibody (LAM-89) [NB600-883]

Applications for Laminin Antibody (LAM-89)

Application
Recommended Usage

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000
Application Notes
Use in ICC/IF reported in scientific literature. Use in IHC-P reported in scientific literature (PMID: 26517388). Can be used for protease-digested formalin-fixed, paraffin embedded human or animal tissue sections. Localizes the basal membrane of blood vessels, epithelium, nerve, and muscle fibers. IHC-P: This concentration was determined by immunoperoxidase staining of protease-digested human tissue. Antigen unmasking is critical in using this product. Optimal dilutions/concentrations should be determined by the end user. indirect ELISA: suitable
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 5 publications using NB600-883 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

15mM Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).

References

1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214

2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567

3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y., . . . Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5

Alternate Names

LAMA, Laminin A chain, laminin subunit alpha-1, laminin, alpha 1, Laminin-1 subunit alpha, Laminin-3 subunit alpha, PTBHS, S-LAM alpha, S-laminin subunit alpha

Entrez Gene IDs

316758 (Rat)

Gene Symbol

LAMA1

Product Documents for Laminin Antibody (LAM-89)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Laminin Antibody (LAM-89)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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