KLHL25 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83974
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HFETVRQSEDFNSLSKDTLLDLISSDELETEDERVVFEAILQWVKHDLEPRKVHLPELLRSVRLALLPSDCLQEAVSSEALLMADERTKLIMDEALRCKTRILQNDGVVTSPCA
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (88%). Reactivity reported in scientific literature (PMID: 23435261)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KLHL25 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: KLHL25 Antibody [NBP1-83974]](https://resources.bio-techne.com/images/products/KLHL25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83974-img0007.jpg "Immunocytochemistry/ Immunofluorescence: KLHL25 Antibody [NBP1-83974]")
Immunocytochemistry/ Immunofluorescence: KLHL25 Antibody [NBP1-83974]
Immunocytochemistry/Immunofluorescence: KLHL25 Antibody [NBP1-83974] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.![Immunohistochemistry-Paraffin: KLHL25 Antibody [NBP1-83974]](https://resources.bio-techne.com/images/products/KLHL25-Antibody-Immunohistochemistry-Paraffin-NBP1-83974-img0002.jpg "Immunohistochemistry-Paraffin: KLHL25 Antibody [NBP1-83974]")
Immunohistochemistry-Paraffin: KLHL25 Antibody [NBP1-83974]
Immunohistochemistry-Paraffin: KLHL25 Antibody [NBP1-83974] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.![KLHL25 Antibody - BSA Free Western Blot: KLHL25 Antibody - BSA Free [NBP1-83974]](https://resources.bio-techne.com/images/products/nbp1-83974_rabbit-polyclonal-klhl25-antibody-84202517195632.jpg "Western Blot: KLHL25 Antibody - BSA Free [NBP1-83974]")
Western Blot: KLHL25 Antibody - BSA Free [NBP1-83974]
Analysis in control (vector only transfected HEK293T lysate) and KLHL25 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).Applications for KLHL25 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KLHL25
Alternate Names
ectodermal-neural cortex 2, ENC-2BTB/POZ KELCH domain protein, ENC2ectoderm-neural cortex protein 2, FLJ12587, FLJ45015, kelch-like 25 (Drosophila), Kelch-like protein 25
Gene Symbol
KLHL25
Additional KLHL25 Products
Product Documents for KLHL25 Antibody - BSA Free
Product Specific Notices for KLHL25 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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