Influenza A H1N1 Antibody - (A/California/14/2009) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30320

Novus Biologicals, part of Bio-Techne
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NBP1-30320
NBP1-30320SS
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Influenza A H1N1 Antibodies »

Product Specifications

Immunogen

A/California/14/2009 (H1N1) virions were used as an immunogen.

Specificity

This antibody detects the following H1N1 proteins: Hemagglutinin (HA) ~75 kDa, Neuraminidase monomer (NA) ~55 kDa, Matrix (M) ~26 kDa and Non-structural Protein monomer (NS) ~26-27 kDa. Reacts with A/New Caledonia/20/99, A/Brisbane/10/2007, A/California/14/2009 and likely reacts with most other isolates.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Influenza A H1N1 Antibody - (A/California/14/2009) - BSA Free

Western Blot: Influenza A H1N1 Antibody(A/California/14/2009) [NBP1-30320]

Western Blot: Influenza A H1N1 Antibody(A/California/14/2009) [NBP1-30320]

Western Blot: Influenza A H1N1 Antibody - (A/California/14/2009), [NBP1-30320] - For the validation Influenza A Virus antibody in WB Assay, 3 ug of intact A/California/14/2009 H1N1 virions were loaded on SDS-PAGE, transferred to a PVDF membrane and detected with NBP1-30320 antibody at a dilution of 1:1000. Goat anti-rabbit secondary antibody was used at 1:10,000 dilution. NBP1-30320 detects: Hemagglutinin (HA) ~75 kDa, Neuraminidase monomer (NA) ~55 kDa, Matrix (M) ~26 kDa and Non-structural Protein monomer (NP) ~26-27 kDa (M and NP often co-migrate as 1 band).
Influenza A H1N1 Antibody - (A/California/14/2009)

Western Blot: Rabbit Polyclonal Influenza A H1N1 Antibody [IMGENEX: IMG-4993] [NBP1-30320] -

Western Blot: Rabbit Polyclonal Influenza A H1N1 Antibody [NBP1-30320] - Image of IAV infected and non-infected A549 whole cell lysates. Image from a verified customer review.

Applications for Influenza A H1N1 Antibody - (A/California/14/2009) - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000
Application Notes
Western Blot detection.

Reviewed Applications

Read 1 review rated 4 using NBP1-30320 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Influenza A H1N1

Influenzavirus A is a genus of the Orthomyxoviridae family of viruses and is a negative sense, single-stranded, segmented RNA virus. Based on the antigenicity of the glycoproteins, influenza A viruses are subdivided into sixteen H (H1-H16) and nine N (N1-N9) subtypes. A standard nomenclature for influenza virus isolates lists the influenza virus type (A or B), the host species (omitted if human origin), the geographical site, year of isolation, and the HA and NA subtype, for example: A/California/14/2009 (H1N1). The main antigenic determinants of influenza A and B viruses are the hemagglutinin (HA) and neuraminidase (NA) transmembrane glycoproteins. Projections of HA and NA cover the surface of the virus particle. NA forms a tetramer with an average molecular weight of 220 kDa (~55 kDa per monomer). The matrix (M) protein of influenza A virus is one of the two group-specific internal proteins of the virion, The non-structural protein (NP) exists as a homeodimer (molecular weight of 52 kDa) consisting of two identical monomers (each ~26 kDa).

Alternate Names

HA, Hemagglutinin

Gene Symbol

HA

Additional Influenza A H1N1 Products

Product Documents for Influenza A H1N1 Antibody - (A/California/14/2009) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Influenza A H1N1 Antibody - (A/California/14/2009) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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