Indole-3-butyric acid Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88846
Key Product Details
Species Reactivity
Non-species specific
Applications
ELISA, Immunoaffinity Purification
Label
Unconjugated
Antibody Source
Polyclonal Chicken IgY
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
BSA-Indole-3-butyric acid conjugate
Specificity
Target: Indole-3-butyric acid (IBA), CAS no.: 133-32-4, Solubility target: Methanol, ethanol.Cross Reactivity Indole-3-actic acid: < 0.1 %
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Description
Novus Biologicals Chicken Indole-3-butyric acid Antibody - BSA Free (NBP2-88846) is a polyclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Applications for Indole-3-butyric acid Antibody - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunoaffinity Purification
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralization of the extract.
Suggested dilution 1:12,800 in indirect ELISA (this titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Plates are coated with 400 ng/ml ovalbumin-conjugated indole-3-butyric acid. HRP-conjugated anti-chicken IgY should be used as a tracer in a 1:8,000 dilution.
Immuno-affinity-chromatography.
Immuno-affinity-chromatography.
Formulation, Preparation, and Storage
Purification
PEG purified
Formulation
0.1 M PBS (pH 7.2)
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Indole-3-butyric acid
Alternate Names
3-Indolebutyric acid, IBA
Additional Indole-3-butyric acid Products
Product Documents for Indole-3-butyric acid Antibody - BSA Free
Product Specific Notices for Indole-3-butyric acid Antibody - BSA Free
Chicken products cannot be exported to Canada.
Store at 4C or -20 C. The working antibody solution is stable for at least 7 days at 4C. Precautions should be taken for storage for longer periods. Problems of long-term stability may occur with highly diluted solutions.
For long storage purposes in solution the addition of sodium azide to 0.02 % is advised with the appropriate precautions of use.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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