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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # RM0263-5J46

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Summary for IL-17E/IL-25 Antibody (RM0263-5J46) - Azide and BSA Free

Immunogen

Mouse recombinant IL-17E/IL-25

Reactivity Notes

Use in Human reported in scientific literature (PMID:34571811). .

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Scientific Data Images for IL-17E/IL-25 Antibody (RM0263-5J46) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: IL-17E/IL-25 Antibody (RM0263-5J46) [NBP2-11677] - Immunofluorescence for IL25-positive cells (red), actin (green) and nuclei (blue) in lung tissue from control mice. The control mice had no experimental manipulation performed. C57BL/6 male mice (Jackson Laboratories, Bar Harbor, Me), 8 to 12 weeks old and weighing 20 to 30 g, were used in this study. Samples were imaged on a FluoView 1000 confocal scanning microscope (Olympus). Imaging conditions were maintained at identical settings within each antibody-labeling experiment with original gating performed using the negative control (no primary antibody). Image courtesy of Center for Biologic Imaging, The University of Pittsburgh

Applications for IL-17E/IL-25 Antibody (RM0263-5J46) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:50-1:200
Application Notes
Use in IHC-P reported in scientific literature (PMID:34571811). IF data from customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-11677 in the following applications:

Published Applications

Read 1 publication using NBP2-11677 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-17E/IL-25

Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C (1) and IL-17E (2). IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis (3), multiple sclerosis (4), and promotion of tumor growth (5). IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated by Shi, et al (6) and Lee, et al. (2) and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8.

Long Name

Interleukin 17E

Alternate Names

IL-25, IL17E, IL25

Gene Symbol

IL25

UniProt

Additional IL-17E/IL-25 Products

Product Documents for IL-17E/IL-25 Antibody (RM0263-5J46) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-17E/IL-25 Antibody (RM0263-5J46) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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