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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1097A

Product Summary for Human XCR1 Alexa Fluor® 488-conjugated Antibody

Immunogen

E. coli-derived recombinant human XCR1
Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
Accession # P46094

Specificity

Detects human XCR1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human XCR1 Alexa Fluor® 488-conjugated Antibody

Detection of XCR1 antibody in CD141+HLA-DR+Human Blood Monocytes antibody by Flow Cytometry.

Detection of XCR1 in CD141+HLA-DR+Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with (A) Mouse Anti-Human HLA-DR Phycoerythrin-Conjugated Monoclonal Antibody (Catalog # FAB4869P), Mouse Anti-Human Thrombomodulin/BDCA-3 (CD141) APC-Conjugated Monoclonal Antibody (Catalog # FAB3947A) and (B) Rabbit Anti-Human XCR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB8571G, filled histogram) or isotype control antibody (Catalog # IC105G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human XCR1 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: CD141+ HLA-DR+ human peripheral blood monocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: XCR1

XCR1, also known as GPR5 and Lymphotactin/SCM-1 (Single Cysteine Motif 1) Receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/Lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, Human Herpes Virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells, NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.

Long Name

Chemokine [C Motif] Receptor 1

Alternate Names

CCXCR1, GPR5

Entrez Gene IDs

2829 (Human); 23832 (Mouse); 301086 (Rat)

Gene Symbol

XCR1

UniProt

Additional XCR1 Products

Product Documents for Human XCR1 Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human XCR1 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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