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Human Ubiquitin+1 Biotinylated Antibody

Bio-Techne includes R&D Systems | Catalog # BAM7032

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BAM7032

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA Detection (Matched Antibody Pair), Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2B Clone # 83426

Product Summary for Human Ubiquitin+1 Biotinylated Antibody

Immunogen

E. coli-derived recombinant human Ubiquitin+1

Specificity

Detects human Ubiquitin+1 in direct ELISAs. This antibody recognizes an epitope from the carboxy-terminal segment that is unique to Ubiquitin+1. It does not cross-react with Ubiquitin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Ubiquitin+1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Ubiquitin+1 (Catalog # 703-UB)

Human Ubiquitin+1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Ubiquitin+1 Antibody (Catalog # MAB7031)
  • Standard: Recombinant Human Ubiquitin+1 Protein, CF (Catalog # 703-UB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ubiquitin+1

Ubiquitin (Ub) is a 6 - 7 kDa polypeptide whose name derives from the observation that Ubiquitin possesses a highly conserved structure that is found in virtually all plant and animal species (1, 2). Ubiquitin is globular in nature, 76 amino acids (aa) in length, contains multiple lysines plus two C‑terminal glycines. In human, there are at least four genes that code for Ubiquitin. Found on human chromosomes 17 (UbB), 2 (UbA-80), 19 (UbA-52) and 12 (UbC), all genes code for a Ubiquitin polymer that undergoes proteolytic processing to generate free, monoubiquitin (3 - 7). In general, about one-half of all Ubiquitin exists in a monomeric form within the cell (8). Ubiquitin can also be added posttranslationally to multiple cell proteins. In conjunction with Ubiquitin ligases E1, 2 and 3, Ubiquitin is covalently attached to amino groups on target molecules via its C-terminal glycines, either at the N-terminus, or on any exposed amino acid that precedes the target's C-terminus (9). Further structural complexity may be added through Ubiquitin binding to Ubiquitin. Depending upon the exact pattern created, cellular proteins possessing UAD (Ub‑associated domain) and UIM (Ub‑interacting motif) sequences will selectively bind ubiquitinated proteins and incorporate them into multiple signaling pathways or regulatory complexes (10, 11). The UbB gene codes for a 229 aa precursor. This precursor contains three contiguous head-to-tail, 76 aa Ub sequences that ends with a C-terminal cysteine. A truncated mutation for UbB, termed Ubiquitin+1, has now been reported, that shows a 20 aa substitution for the last Gly of the first Ub sequence, generating a 95 aa polypeptide (12). Although a mutation, this molecule is apparently commonly expressed (13). At low levels of expression, it is degraded in a proteosome-dependent manner. At high levels, it overwhelms the proteosome system and accumulates, inhibiting proteosome activity (13). This is suggested to contribute to pathology associated with polyglutamine diseases (14).  

 

References

  1. Rechsteiner, M. (1987) Annu. Rev. Cell Biol. 3:1.
  2. Hershko, A. and A. Ciechanover (1998) Annu. Rev. Biochem. 67:425.
  3. Kim, N-S. et al. (1998) J. Biochem. 124:35.
  4. Lund, P.K. et al. (1985) J. Biol. Chem. 260:7609.
  5. Redman, K.L. and M. Rechsteiner (1989) Nature 338:438.
  6. Monia, B.P. et al. (1989) J. Biol. Chem. 264:4093.
  7. Wiborg, O. et al. (1985) EMBO J. 4:755.
  8. Agell, N. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3693.
  9. Ciechanover, A. and R. Ben-Saadon (2004) Trends Cell Biol. 14:103.
  10. Kim, H. T. et al. (2007) J. Biol. Chem. 282:17375.
  11. Ye, Y. and M. Rape (2009) Nat. Rev. Mol. Cell Biol. 10:755.
  12. van Leeuwen, F.W, et al. (1998) Science 279:242.
  13. van Tijn, P. et al. (2007) J. Cell Sci. 120:1615.
  14. de Pril, R. et al. (2004) Hum. Mol. Genet. 13:1803.

Long Name

frame shift mutant

Alternate Names

FLJ25987, MGC8385, polyubiquitin B, polyubiquitin-B, RPS27A, UBA52, UBC, ubiquitin B

Entrez Gene IDs

7314 (Human); 298693 (Rat)

Gene Symbol

UBB

Additional Ubiquitin+1 Products

Product Documents for Human Ubiquitin+1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Ubiquitin+1 Biotinylated Antibody

For research use only

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