Detects human Tryptase alpha/TPS1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross‑reactivity with recombinant human (rh) TPSB2 is observed and 10% cross-reactivity with rhTPSG1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Tryptase alpha/TPS1 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Tryptase alpha/TPS1, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Tryptase alpha/TPS1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase alpha/TPS1

Tryptases are serine proteases with trypsin-like specificity. Together with chymases and Cathepsin G, tryptases are important players in mast cell mediation of inflammatory and allergic responses. Tryptase beta-1, also known as mast cell protease 7 (MCPT7), exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of a diverse array of protease inhibitors in plasma. Tryptase epsilon, also known as brain-specific serine protease 4 (BSSP-4) and brain serine protease 2 (BSP-2), is encoded by the PRSS22 gene and preferentially expressed in epithelium-rich tissues such as lung and eye. Tryptase gamma-1, also called transmembrane tryptase, is encoded by TPSG1, one of many serine protease genes clustered on human chromosome 16p13.3.

Alternate Names

alpha-Tryptase, TPS1, TPSAB1, Tryptase 1

Entrez Gene IDs

7177 (Human)

Gene Symbol

TPSAB1

UniProt

AAA86934

Additional Tryptase alpha/TPS1 Products

Product Documents for Human Tryptase alpha/TPS1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Tryptase alpha/TPS1 Antibody

For research use only

Citations
Reviews

Human Tryptase alpha/TPS1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2370

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Tryptase alpha/TPS1 Antibodies »

Product Specifications

Immunogen

Specificity