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Human TLR4 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB14784

Catalog #
Size / Price

Key Product Details

Species Reactivity







Antibody Source

Monoclonal Mouse IgG2A Clone # 1039443

Product Summary for Human TLR4 Antibody


Mouse myeloma cell line NS0-derived human TLR4
Accession # O00206


Detects human TLR4 in direct ELISAs.







Scientific Data Images for Human TLR4 Antibody

TLR4 in Human Tonsil.

TLR4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human TLR4 Monoclonal Antibody (Catalog # MAB14784) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human TLR4 Antibody

Recommended Usage


5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB14784 in the following applications:

Formulation, Preparation, and Storage


Protein A or G purified from hybridoma culture supernatant


Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR4

TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll‑Like Receptor family of pathogen pattern recognition molecules. The complex of TLR4 with MD‑2 functions as a critical receptor for bacterial endotoxin/lipopolysaccharide (LPS) (1-3). Mature human TLR4 consists of a 608 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 187 aa cytoplasmic domain. TLR4 contains 21 leucine rich repeats in its ECD and one cytoplasmic Toll/IL‑1 receptor (TIR) domain (4). The ECD of human TLR4 shares approximately 25% aa sequence identity with other human TLRs and 60%-74% aa sequence identity with bovine, equine, feline, mouse, rat, and porcine TLR4. On monocytes, macrophages, dendritic cells, and B cells, MD-2 expression is required for cell surface localization of TLR4 and for optimal LPS-induced TLR4 signaling (5-8). MD-2 also forms soluble disulfide‑linked homo‑oligomers which can interact with TLR4 (6). Through a domain separate from its TLR4-binding domain, MD-2 extracts LPS from circulating CD14-LPS complexes and carries the LPS into a ternary complex with TLR4 (9-11). The interaction of MD-2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (2, 12). Increased levels of plasma MD-2 in septic shock patients sensitizes MD-2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (13).


  1. Miyake, K. (2004) Semin. Immunol. 16:11.
  2. Verstrepen, L. et al. (2008) Cell. Mol. Life Sci. 65:2964.
  3. Erridge, C. (2010) J. Leukoc. Biol. 87:989.
  4. Medzhitov, R. et al. (1997) Nature 388:394.
  5. Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
  6. Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. 98:12156.
  7. Akashi, S. et al. (2000) J. Immunol. 164:3471.
  8. Nagai, Y. et al. (2002) Nat. Immunol. 3:667.
  9. Re, F. and J.L. Strominger (2003) J. Immunol. 171:5272.
  10. Kennedy, M.N. et al. (2004) J. Biol. Chem. 279:34698.
  11. Gioannini, T.L. et al. (2004) Proc. Natl. Acad. Sci. 101:4186.
  12. Saitoh, S. et al. (2004) J. Endotoxin Res. 10:257.
  13. Pugin, J. et al. (2004) Blood 104:4071.

Long Name

Toll-like Receptor 4

Alternate Names


Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Gene Symbol



Additional TLR4 Products

Product Documents for Human TLR4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TLR4 Antibody

For research use only