Human STRO-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1038

Discontinued Product
MAB1038 has been discontinued. View all STRO-1 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Porcine, Canine, Deer, Feline

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Cell Selection, MACS

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # STRO-1

View all STRO-1 Antibodies »

Product Specifications

Immunogen

Human CD34+ bone marrow cells

Specificity

Detects human STRO-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human STRO-1 Antibody

STRO-1 antibody in Human MG-63 Cell Line by Immunocytochemistry (ICC).

STRO‑1 in Human MG‑63 Cell Line.

STRO-1 was detected in immersion fixed MG-63 human osteosarcoma cell line using Human STRO-1 Monoclonal Antibody (Catalog # MAB1038) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; NL019), and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human STRO-1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human long term bone marrow culture and immersion fixed embryonic mouse parietal endoderm cells (E10.5)

Reviewed Applications

Read 9 reviews rated 4.2 using MAB1038 in the following applications:

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STRO-1

STRO-1 is a cell surface protein expressed by bone marrow stromal cells and erythroid precursors. The frequency of colony forming units-fibroblasts (CFU-F) was enriched 100-fold in the STRO-1+/Glycophorin A- population from bone marrow cells (1). The subset of marrow cells that expresses the STRO-1 antigen is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts, and chondrocytes (2).

References

  1. Simmons, P.J. and B. Torok-Storb (1991) Blood 78:55.
  2. Dennis, J.E. et al. (2002) Cells Tissues Organs 170:73.

Long Name

A Stromal Cell Precursor Surface Antigen

Alternate Names

STRO1

Additional STRO-1 Products

Product Documents for Human STRO-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human STRO-1 Antibody

For research use only

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