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Human Siglec-11 PE-conjugated Antibody

Bio-Techne includes R&D Systems | Catalog # FAB3258P

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FAB3258P

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG

Product Summary for Human Siglec-11 PE-conjugated Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Siglec-11
Asn17-His543 (Glu84Ala, Lys145Gln)
Accession # Q96RL6

Specificity

Detects human Siglec-11 in direct ELISAs and Western blots. In direct ELISA and Western blots, approximately 50% cross-reactivity with recombinant human (rh) Siglec-10 is observed and less than 5% cross-reactivity with rhSiglec-2, -3, -5, -6, -7, -9 and recombinant mouse Siglec-F is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Siglec-11 PE-conjugated Antibody

Detection of Siglec-11 antibody in Human Peripheral Blood Mononuclear Cell Monocytes and Granulocytes antibody by Flow Cytometry.

Detection of Siglec‑11 in Human Peripheral Blood Mononuclear Cell Monocytes and Granulocytes by Flow Cytometry.

Human peripheral blood mononuclear cell monocytes and granulocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Goat Anti-Human Siglec-11 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3258P) or (B) Normal Goat IgG Phycoerythrin Control (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Siglec-11 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cell monocytes and granulocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using FAB3258P in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Siglec-11

Siglecs (Sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-set domain which mediates sialic acid binding, followed by a varying numbers of Ig-like C2-set domains. Siglecs‑3 and -5 through -13 constitute the CD33/Siglec-3 related group, which are defined by their sequence homology and differential expression in the hematopoietic system. Mature human Siglec-11 is an 85-90 kDa, type I transmembrane glycoprotein that consists of a 534 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 114 aa cytoplasmic domain. The ECD contains one Ig-like V-set domain, and three Ig-like C2-set domains. The cytoplasmic domain contains two Immunoreceptor Tyrosine-based Inhibitory Motifs (ITIMs). A splice variant of Siglec-11 has a deletion of nearly 100 aa in the extracellular juxtamembrane region. Among siglecs, the ECD of Siglec-11 is most closely related to that of Siglec-10 (82% aa sequence identity). The cytoplasmic domains of these proteins are only 20% identical. Siglec-11 is closely related to the pseudogenes Siglec-14 and Siglec-16. Human Siglec-11 shares 90%‑96% aa sequence identity with Siglec-11 from great apes. Rodent orthologs of Siglec-11 have not been identified. In human, Siglec-11 is expressed in tissue macrophages, brain microglia, and inflammatory site monocytes. Strong microglial expression is specific to humans, as it is less prominent or absent in chimpanzees and orangutans. Siglec-11 forms 180 kDa disulfide-linked dimers. It shows a strong binding preference for sialic acid in alpha2-8 linkage which is unusual for siglecs. Notably, this applies to polysialyated NCAM residues on neurons where Siglec-11 activation imparts neuroprotection towards neurons in response to neuroinflammation.

Long Name

Sialic Acid Binding Ig-like Lectin 11

Alternate Names

Siglec11

Entrez Gene IDs

114132 (Human)

Gene Symbol

SIGLEC11

UniProt

Additional Siglec-11 Products

Product Documents for Human Siglec-11 PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Siglec-11 PE-conjugated Antibody

For research use only

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