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Human Siglec-10 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB2130

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MAB2130
MAB2130-SP

Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 265817

Product Summary for Human Siglec-10 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Siglec-10
Met17-Thr546
Accession # Q96LC7

Specificity

Detects human Siglec-10 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Siglec-11 is observed. In Western blots (reducing conditions), approximately 25% with rhSiglec-11 is observed. In direct ELISAs and Western blots, no cross-reactivity with rhSiglec-2, -3, -5, -7, -9, or recombinant mouse Siglec-F is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Siglec-10 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Siglec-10 Fc Chimera (Catalog # 2130-SL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-10

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5‑11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1‑3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V-type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5‑8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5‑7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is up‑regulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec‑10 binds sialated proteins and lipids in alpha2,3 or alpha2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (7). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP‑2 to propagate inhibitory signals (5, 9).

References

  1. Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
  2. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  3. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
  4. Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
  5. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
  6. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900.
  7. Li, N. et al. (2001) J. Biol. Chem. 276:28106.
  8. Munday, J. et al. (2001) Biochem. J. 355:489.
  9. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
  10. Aizawa, H. et al. (2003) Genomics 82:521.
  11. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.

Long Name

Sialic Acid Binding Ig-like Lectin 10

Alternate Names

Siglec10, SLG2

Entrez Gene IDs

89790 (Human)

Gene Symbol

SIGLEC10

UniProt

Additional Siglec-10 Products

Product Documents for Human Siglec-10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Siglec-10 Antibody

For research use only

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