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Human RARRES3 Antibody

Bio-Techne includes R&D Systems | Catalog # AF6139

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AF6139
AF6139-SP

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Product Summary for Human RARRES3 Antibody

Immunogen

E. coli-derived recombinant human RARRES3
Asp12-Lys134
Accession # Q9UL19

Specificity

Detects human RARRES3 in direct ELISAs.  In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) RARRES1 and rhLatexin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human RARRES3 Antibody

RARRES3 antibody in Raji Human Cell Line by Immunocytochemistry (ICC).

RARRES3 in Raji Human Cell Line.

RARRES3 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Human RARRES3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human RARRES3 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RARRES3

RARRES3 (Retinoic acid receptor responder 3; also Tig3) is an 18-20 kDa member of the lecithin retinol acyltransferase family of molecules. It is widely expressed, particularly in keratinocytes, and is considered to have multiple functions. These include phospholipase A activity and an ability to both activate type I transglutaminase, and inhibit Ras activation Human RARRES3 is 164 amino acids (aa) in length. It contains a catalytic residue at Cys113, and a hydrophobic membrane-anchoring domain between aa 134-164. Within the cell, RARRES3 appears to be equally distributed between membrane and soluble fractions. RARRES3 is also reported to form higher order multimers. One potential isoform exists that shows a 26 aa substitution for aa 131-164. No meaningful structural ortholog of RARRES3 exists in rodent.

Long Name

Retinoic Acid Receptor Responder [Tazarotene-induced] 3

Alternate Names

HRASLS4, TIG3

Entrez Gene IDs

5920 (Human)

Gene Symbol

PLAAT4

UniProt

Additional RARRES3 Products

Product Documents for Human RARRES3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human RARRES3 Antibody

For research use only

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