Human Phospho-STAT2 (Y689) Antibody
Bio-Techne includes R&D Systems | Catalog # AF2890
Discontinued Product
AF2890 has been discontinued and is replaced by
MAB2890.
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Summary for Human Phospho-STAT2 (Y689) Antibody
Immunogen
Phosphopeptide containing human STAT2 Y689 site
Specificity
Detects human STAT2 when phosphorylated at Y689 in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-STAT2 (Y689) Antibody
Detection of Phospho-STAT2 in IFN-alpha -treated Daudi Human Cell Line by Flow Cytometry.
Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN-a (Catalog # 11100-1, dark orange filled histogram) for 20 minutes, then stained with Rabbit Anti-Human Phospho-STAT2 (Y689) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2890) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Detection of Human Phospho-STAT2 (Y689) by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-a2a (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y689) Antigen Affinity-purified Polyclonal Antibody (Catalog AF2890), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT2 (Y689) at approximately 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human Phospho-STAT2 (Y689) by Simple WesternTM.
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT2 (Y689) at approximately 111 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y689) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2890). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Phospho-STAT2 (Y689) Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Daudi human Burkitt's lymphoma cell line treated with IFN-alpha, fixed with paraformaldehyde and permeabilized with methanol
Sample: Daudi human Burkitt's lymphoma cell line treated with IFN-alpha, fixed with paraformaldehyde and permeabilized with methanol
Simple Western
5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with 500 U/mL Recombinant Human IFN‑ alphaA (Catalog # 11100-1)
Sample: Daudi human Burkitt's lymphoma cell line treated with 500 U/mL Recombinant Human IFN‑ alphaA (Catalog # 11100-1)
Western Blot
0.5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN-alpha 2a
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN-alpha 2a
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: STAT2
N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y689 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.
Long Name
Signal Transducer and Activator of Transcription 2
Alternate Names
interferon alpha induced transcriptional activator, ISGF-3, MGC59816, p113, signal transducer and activator of transcription 2, signal transducer and activator of transcription 2, 113kD, signal transducer and activator of transcription 2, 113kDa, STAT113
Gene Symbol
STAT2
Additional STAT2 Products
Product Documents for Human Phospho-STAT2 (Y689) Antibody
Product Specific Notices for Human Phospho-STAT2 (Y689) Antibody
For research use only
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