Human Pannexin-3 Antibody

Catalog # MAB8169 | R&D Systems, Inc. a Bio-Techne Brand
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MAB8169
MAB8169-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 788905

Product Summary for Human Pannexin-3 Antibody

Immunogen

E. coli-derived recombinant human Pannexin-3
Tyr289-Thr384
Accession # Q96QZ0

Specificity

Detects human Pannexin-3 in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Pannexin-3 Antibody

Detection of Human Pannexin-3 antibody by Western Blot.

Detection of Human Pannexin-3 by Western Blot.

Western blot shows lysates of SW1353 human chondrosarcoma cell line and Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pannexin-3 Monoclonal Antibody (Catalog # MAB8169) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pannexin-3 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Pannexin-3 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: SW1353 human chondrosarcoma cell line and Bowes human melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using MAB8169 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pannexin-3

Pannexin-3 (PANX3) is an approximately 45 kDa member of the Pannexin family of four-transmembrane channel proteins with a conserved pattern of cysteines. It is expressed in skin, cochlea, and developing hard tissues including cartilage and bone. In osteoblasts, it forms Ca2+ channels in the endoplasmic reticulum and promotes osteogenic differentiation. It also forms gap junction channels that pass Ca2+ between cells, and ATP-releasing hemichannels in the plasma membrane. Within aa 289-384 (in the C-terminal cytoplasmic domain), human Pannexin-3 shares 95% and 99% aa sequence identity with mouse and rat Pannexin-3, respectively.

Alternate Names

Pannexin3, PANX3, PX3

Entrez Gene IDs

116337 (Human); 208098 (Mouse); 315567 (Rat)

Gene Symbol

PANX3

UniProt

Q96QZ0

Product Documents for Human Pannexin-3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pannexin-3 Antibody

For research use only

Citations for Human Pannexin-3 Antibody (1)

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