Human Netrin-G1a Alexa Fluor® 750-conjugated Antibody

Name: Human Netrin-G1a Alexa Fluor® 750-conjugated Antibody
Brand: R&D Systems
SKU: FAB10019S

R&D Systems, part of Bio-Techne | Catalog # FAB10019S

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Key Product Details

Species Reactivity:	Human
Immunogen:	Human embryonic kidney cell, HEK293-derived human Netrin-G1a His29-Gly503 Accession # Q9Y212
Applications:	Flow Cytometry
Label:	Alexa Fluor 750 (Excitation=749 nm, Emission=775 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 1010035
Specificity:	Detects human Netrin-G1a in direct ELISAs.

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Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Flow Cytometry	0.25-1 µg/10⁶ cells	HEK293 Human Cell Line Transfected with Human Netrin‑G1a and U87-MG human glioblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: Netrin-G1a

NTNG-1, known as Netrin-G1 or alternatively Laminet-1 (Gene name: NTNG1), is a member of the UNC-6/Netrin family of proteins. Human Netrin-G1 is a 55 kDa protein and shares 100% homology with its crab-eating macaque, 98% homology with its rat homolog, and 97% homology with its mouse homolog. Netrin-G1 is synthesized as a 539 amino acid (aa) precursor with a 28 aa signal sequence, a 251 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, a GPI (glycosylphosphatidylinisotol) anchor (Ser510), and a C-terminal 28 aa propeptide that is removed in mature Netrin-G1 (1, 3, 4). Netrin-G1 interacts with its ligand, Netrin-G ligand 1 (NGL1) to regulate neuron growth and patterning, axonal subdendritic differentiation, and synapse formation throughout development (1, 2, 4, 6). Abnormal expression of Netrin-G1 via SNPs (single nucleotide polymorphisms) have been implicated in pathogenesis for schizophrenia (5). Netrin-G1 has widespread expression only in vertebrates, occurring in olfactory mitral cells, cells of the inferior colliculus (hearing), dorsal thalamus (behavior), and cells of the deep cerebellar nuclei and inferior olive (motion) (3).

Item	Value
References	Seiradake, E. et al. (2011) EMBO J. 30:4479. Song, Y. et al. (2013) J Cell Sci. 126:4926. Nakashiba, T. et al. (2000) J Neuroscience. 20:6540. Matsukawa, H. et al. (2014) J Neuroscience. 34:15779. Zhu, Y. et al. (2011) J Genet. 90:499. Nishimura-Akiyoshi, S. et al. (2007) Neuroscience. 104:14801.
Entrez Gene IDs	22854 (Human); 80883 (Mouse)
Alternate Names	Lmnt1, NTG1, NTNG1, Netrin-G1a, NetrinG1a

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human Netrin-G1a Alexa Fluor® 750-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB10019S

Key Product Details

Applications

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: Netrin-G1a

Product Specific Notices

Customer Reviews

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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