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Human N‑Cadherin Antibody

Catalog # MAB13882 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Species Reactivity



CyTOF-ready, Flow Cytometry



Antibody Source

Monoclonal Mouse IgM Clone # 691701

Product Summary for Human N‑Cadherin Antibody


Mouse myeloma cell line NS0-derived recombinant human N-Cadherin
Accession # P19022


Detects human N-Cadherin in direct ELISAs. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse N-Cadherin is observed and no cross-reactivity with recombinant human (rh) E-Cadherin or rhCadherin-4/R-Cadherin is observed.







Scientific Data Images for Human N‑Cadherin Antibody

Detection of N-Cadherin antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of N-Cadherin in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human N-Cadherin Monoclonal Antibody (Catalog # MAB13882, filled histogram) or isotype control antibody mouse IgM (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).

Applications for Human N‑Cadherin Antibody

Recommended Usage


Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Protein A or G purified from hybridoma culture supernatant


Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: N-Cadherin

N‑Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130‑135 kDa member of the "classical" (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N‑Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN‑Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15‑20 kDa prodomain (aa 26‑159) is cleaved by proprotein convertase, and the mature molecule is transported to the surface. Alternatively, on neurons, proN‑Cadherin may first appear on the surface, with cleavage occurring at the time of synaptogenesis. Cleavage appears necessary for homophilic interaction as presence of the prodomain is suggested to negatively regulate oligomer formation. Over the entire prodomain, the human N‑Cadherin proregion shares 87% aa identity with the mouse N‑Cadherin proregion.

Long Name

Neural Cadherin

Alternate Names

Cadherin-2, CD325, CDH2, NCadherin

Entrez Gene IDs

1000 (Human); 12558 (Mouse); 83501 (Rat)

Gene Symbol



Product Documents for Human N‑Cadherin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human N‑Cadherin Antibody

For research use only

Reconstitution Calculator

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