Human/Mouse/Rat Calcineurin B Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1348U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 212306
Product Specifications
Immunogen
E. coli-derived recombinant human Calcineurin B
Met1-Val170
Accession # P63098
Met1-Val170
Accession # P63098
Specificity
Detects endogenous human, mouse, and rat Calcineurin B.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Calcineurin B
Background
Calcineurin, also called Protein Phosphatase 2B,
PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is
an enzyme that dephosphorylates serine and
threonine residues in proteins. It is a heterodimer of
a 59,000 dalton catalytic A subunit and a
1,900 dalton regulatory B subunit that is activated
by the binding of calcium ions and calmodulin (1).
Calcineurin is expressed in many tissues, but its
levels are highest in the brain, where it may play a
role in learning and memory (2). It has many
substrates, including NFAT, a transcription factor
that is activated by dephosphorylation (3).
Complexes of the immunosuppressants cyclosporin
and FK506 with immunophilin proteins such as
cyclophilin and FKBP12 are potent and specific
inhibitors of Calcineurin activity (4). Alterations in
Calcineurin activity are suspected to play a role in
cardiac hypertrophy (5) and graft versus host
disease in organ transplantation (6).
References:
1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.
2. Zeng H. et al. (2001) Cell 107:617.
3. Okamura, H. et al. (2000) Molecular Cell 6:539.
4. Liu, J. et al. (1992) Biochemistry 31:3896.
5. Molkentin, J.D. (2000) Circulation Research 87:731.
6. Sanquer, S.A. et al. (2004) Transplantation 77:854.
Alternate Names
CALNB1, Cnb1, MCIP1, PP2B beta 1, PPP3R1
Gene Symbol
PPP3R1
UniProt
Additional Calcineurin B Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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