Human/Mouse Frizzled‑7 Antibody
Catalog # MAB1981 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
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Antibody Source
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Product Summary for Human/Mouse Frizzled‑7 Antibody
Immunogen
Gln33-Leu185
Accession # Q61090
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse Frizzled‑7 Antibody
Frizzled‑7 in HEK293 Human Cell Line.
Frizzled-7 was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human/Mouse Frizzled-7 Monoclonal Antibody (Catalog # MAB1981) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse Frizzled‑7 Antibody
CyTOF-ready
Flow Cytometry
Sample: Retinoic acid‑treated D3 mouse embryonic stem cell line
Immunocytochemistry
Sample: Immersion fixed HEK293 human embryonic kidney cell line
Reviewed Applications
Read 3 reviews rated 5 using MAB1981 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-7
References
- Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
- Vincan, E. et al. (2007) Front. Biosci. 12:4558.
- Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
- Medina, A. et al. (2000) Mech. Dev. 92:227.
- Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
- Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
- Melchior, K. et al. (2008) Biol. Chem. 389:897.
- Vincan, E. et al. (2007) Oncogene 26:2340.
Alternate Names
Gene Symbol
UniProt
Product Documents for Human/Mouse Frizzled‑7 Antibody
Product Specific Notices for Human/Mouse Frizzled‑7 Antibody
For research use only
Citations for Human/Mouse Frizzled‑7 Antibody (4)
Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse Frizzled‑7 Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryE Flores-Her et al. (2020-04-10), Canonical and non-canonical Wnt signaling are simultaneously activated by Wnts in colon cancer cells Cell. Signal., 2020-072(0):109636.
PMID: 32283254 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryWu DT et al. (2012-04-20), Antibody-directed lentiviral gene transduction for live-cell monitoring and selection of human iPS and hES cells. PLoS ONE, 2012-07(4):e34778.
PMID: 22536330 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryVijayaragavan K et al. (2009-03-06), Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells. Cell Stem Cell, 2009-04(3):248-62.
PMID: 19265664 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryBaksh D et al. (2007-08-01), Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. J. Cell. Biochem., 2007-0101(5):1109-24.
PMID: 17546602
There are no citations that match your criteria.
Customer Reviews for Human/Mouse Frizzled‑7 Antibody (3)
Customer Images
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Application: Western BlotSample Tested: HEK293 human embryonic kidney cell lineSpecies: Human and human eyesVerified Customer | Posted 08/17/20211:1000 dilution worked very well
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Application: Frizzled 7 was detected in the limbusSample Tested: mouse eyeSpecies: MouseVerified Customer | Posted 08/06/2021dilution 1:100-1:200
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: limbal stem cellsSpecies: Mouse and HumanVerified Customer | Posted 07/21/2021It works very well with 1:200 dilution.
There are no reviews that match your criteria.
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