Human LIMPII/SR-B2 Antibody

Name: Human LIMPII/SR-B2 Antibody
Brand: R&D Systems
SKU: AF1966

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1966

Citations (10)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human LIMPII lumenal loop Arg27-Thr432 Accession # Q14108
Applications:	ELISA Capture, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human LIMPII in ELISAs and Western blots. In sandwich immunoassays, approximately 6% cross-reactivity with recombinant mouse LIMPII is observed and less than 0.3% cross-reactivity with recombinant human (rh) SR-B1 and rhCD36 is observed.

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Applications for Human LIMPII/SR-B2 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human LIMPII/SR-B2 Fc Chimera (Catalog # 1966-LM)

Human LIMPII/SR-B2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Human LIMPII/SR-B2 Lumenal Loop Biotinylated Antibody (Catalog # BAF1966)

Standard: Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF (Catalog # 1966-LM)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: LIMPII/SR-B2

LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and as CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1 - 3). SR-B/CD36 family members are type III integral membrane proteins with an N- as well as a C-terminal cytoplasmic tail, and a large extracellular (or lumenal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is widely expressed on all tissues and cell types so far examined. It is also expressed on the surface of activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII-thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation (1). Overexpression of LIMPII causes an enlargement of early endosomes and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).

Item	Value
References	Crombie, R. and R. Silverstein (1998) J. Biol. Chem. 273:4855. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. Eskelinen, E-L. et al. (2003) Trends in Cell Biology 13:137. Kuronita, T. et al. (2002) J. Cell Sci. 115:4117. Gamp, A-C. et al. (2003) Human Molecular Genetics 12:631.
Long Name	Lysosomal Integral Membrane Protein II
Entrez Gene IDs	950 (Human); 12492 (Mouse)
Alternate Names	AMRF, CD36 antigen, CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II), CD36L2, CD36L2LIMP II, HLGP85, LGP85, LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2, LIMPII, LIMPIICD36 antigen-like 2, LPG85, Lysosome membrane protein II, SCARB2, SR-B2, SR-BII, SRB2, Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein, lysosome membrane protein 2, scavenger receptor class B, member 2

Citations for Human LIMPII/SR-B2 Antibody (10)

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Citations are publications that use Bio-Techne products. Selected citations for Human LIMPII/SR-B2 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

J Singh et al. (2022), Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo Nature Communications, 13(1):6212.
PMID: 36266287
Species: Human
Sample Types: Tissue Homogenates

GF Jang et al. (2021), Proteomics of Primary Uveal Melanoma: Insights into Metastasis and Protein Biomarkers Cancers, 13(14).
PMID: 34298739
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

J Yan et al. (2020), Involvement of VCP/UFD1/Nucleolin in the viral entry of Enterovirus A species Virus Res., 283(0):197974.
PMID: 32289342
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

K Kobayashi et al. (2020), Heparan sulfate attachment receptor is a major selection factor for attenuated enterovirus 71 mutants during cell culture adaptation PLoS Pathog., 16(3):e1008428.
PMID: 32187235
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

QQ He et al. (2018), Fibronectin facilitates EV71 infection by mediating its entry J. Virol., 0(0).
PMID: 29467312
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

X Zhang et al. (2017), The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection Protein Cell, 0(0).
PMID: 28447294
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

J Jin et al. (2017), Transcriptome analysis reveals dynamic changes in coxsackievirus A16 infected HEK 293T cells BMC Genomics, 18(0):933.
PMID: 28198671
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

(2016), Amphotericin B Inhibits Enterovirus 71 Replication by Impeding Viral Entry Sci Rep, 6(0):33150.
PMID: 27608771
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Leong et al. (2015), The role of Misshapen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human enterovirus 71. PLoS Pathog, 11(3):e1004686.
PMID: 25747578
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Yamayoshi S et al. (2009), Scavenger receptor B2 is a cellular receptor for enterovirus 71. Nat. Med., 15(7):798-801.
PMID: 19543282

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Human LIMPII/SR-B2 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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