Human KGF/FGF‑7 Antibody
Catalog # AF-251-NA | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for Human KGF/FGF‑7 Antibody
Immunogen
Cys32-Thr194
Accession # Q6FGV5
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human KGF/FGF‑7 Antibody
Cell Proliferation Induced by KGF/FGF‑7 and Neutralization by Human KGF/FGF‑7 Antibody.
Recombinant Human KGF/FGF‑7 (Catalog # 251-KG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human KGF/FGF‑7 (125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA). The ND50 is typically 6-12 µg/mL.KGF/FGF‑7 in Human Placenta.
KGF/FGF-7 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using Goat Anti-Human KGF/FGF-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human KGF/FGF‑7 Antibody
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human placenta
Western Blot
Sample: Recombinant Human KGF/FGF‑7 (Catalog # 251-KG)
Neutralization
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KGF/FGF-7
KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1‑4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta-trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.
References
- Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
- Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
- Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
- Mason, I.J. et al. (1994) Mech. Dev. 45:15.
- Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
- Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
- Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
- Qiao, J. et al. (1999) Development 126:547.
- Guo, L. et al. (1996) Genes Dev. 10:165.
- de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
- Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
- Belleudi, F. et al. (2007) Traffic 8:1854.
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Product Documents for Human KGF/FGF‑7 Antibody
Product Specific Notices for Human KGF/FGF‑7 Antibody
For research use only
Citations for Human KGF/FGF‑7 Antibody (6)
Citations are publications that use Bio-Techne products. Selected citations for Human KGF/FGF‑7 Antibody include:
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Species: Human
Sample Types: Whole Tissue,Cell Lysates
Applications: Co-Immunoprecipitation,IHCDB Roldán et al. (2020-05-19), PLAC1 is essential for FGF7/FGFRIIIb-induced Akt-mediated cancer cell proliferation Oncotarget, 2020-011(20):1862-1875.
PMID: 32499871 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotH Yabuki et al. (2018-04-30), Human Multilineage-differentiating Stress-Enduring Cells Exert Pleiotropic Effects to Ameliorate Acute Lung Ischemia-Reperfusion Injury in a Rat Model Cell Transplant, 2018-00(0):9636897187616.
PMID: 29707971 -
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-PNadworny PL et al. (2010-02-19), Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. J Inflamm (Lond), 2010-07(0):13.
PMID: 20170497 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationSkibinski G et al. (2007-03-23), Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-0293(1):L69-76.
PMID: 17384084 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotLee JH et al. (2006-03-01), Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci., 2006-047(3):864-72.
PMID: 16505018 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotGroschl M et al. (2005-04-01), Salivary leptin induces increased expression of growth factors in oral keratinocytes. J. Mol. Endocrinol., 2005-034(2):353-66.
PMID: 15821102
There are no citations that match your criteria.
Customer Reviews for Human KGF/FGF‑7 Antibody (2)
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Application: Western BlotSample Tested: See PMID 24046361Species: MouseVerified Customer | Posted 01/07/2015
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Application: ELISASample Tested: See PMID 23582717Species: HumanVerified Customer | Posted 01/07/2015
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