Human KGF/FGF‑7 Antibody

Name: Human KGF/FGF‑7 Antibody
Brand: R&D Systems
SKU: AF-251-NA
Rating: 4 (2 reviews)

Catalog # AF-251-NA | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (6)

Product Datasheet / COA / SDS

100% Guarantee

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human KGF/FGF‑7 Antibody

Immunogen

E. coli-derived recombinant human KGF/FGF‑7
Cys32-Thr194
Accession # Q6FGV5

Specificity

Detects human KGF/FGF‑7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse FGF-7 is observed, less than 20% cross-reactivity with recombinant human (rh) FGF-9 is observed, and less than 10% cross‑reactivity with rhFGF-4 and rhFGF-9 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human KGF/FGF‑7 Antibody

Cell Proliferation Induced by KGF/FGF‑7 and Neutralization by Human KGF/FGF‑7 Antibody.

Recombinant Human KGF/FGF‑7 (Catalog # 251-KG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human KGF/FGF‑7 (125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA). The ND₅₀ is typically 6-12 µg/mL.

KGF/FGF‑7 in Human Placenta.

KGF/FGF-7 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using Goat Anti-Human KGF/FGF-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human KGF/FGF‑7 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Western Blot

0.1 µg/mL
Sample: Recombinant Human KGF/FGF‑7 (Catalog # 251-KG)

Neutralization

Measured by its ability to neutralize KGF/FGF‑7-induced proliferation in the 4MBr‑5 rhesus monkey epithelial cell line. The Neutralization Dose (ND₅₀) is typically 6-12 µg/mL in the presence of 125 ng/mL Recombinant Human KGF/FGF‑7.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KGF/FGF-7

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1‑4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta-trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

References

Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
Mason, I.J. et al. (1994) Mech. Dev. 45:15.
Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
Qiao, J. et al. (1999) Development 126:547.
Guo, L. et al. (1996) Genes Dev. 10:165.
de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
Belleudi, F. et al. (2007) Traffic 8:1854.

Long Name

Keratinocyte Growth Factor

Alternate Names

FGF-7, FGF7, HBGF-7, HBGF7

Entrez Gene IDs

403915 (Canine); 2252 (Human)

Gene Symbol

FGF7

UniProt

Q6FGV5

Product Documents for Human KGF/FGF‑7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human KGF/FGF‑7 Antibody

For research use only

Citations
Reviews

Citations for Human KGF/FGF‑7 Antibody (6)

Showing 1 - 6 of 6 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human KGF/FGF‑7 Antibody include:

Species: Human
Sample Types: Whole Tissue,Cell Lysates
Applications: Co-Immunoprecipitation,IHC

DB Roldán et al. (2020-05-19), PLAC1 is essential for FGF7/FGFRIIIb-induced Akt-mediated cancer cell proliferation Oncotarget, 2020-011(20):1862-1875.
PMID: 32499871
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

H Yabuki et al. (2018-04-30), Human Multilineage-differentiating Stress-Enduring Cells Exert Pleiotropic Effects to Ameliorate Acute Lung Ischemia-Reperfusion Injury in a Rat Model Cell Transplant, 2018-00(0):9636897187616.
PMID: 29707971
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-P

Nadworny PL et al. (2010-02-19), Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. J Inflamm (Lond), 2010-07(0):13.
PMID: 20170497
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Skibinski G et al. (2007-03-23), Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-0293(1):L69-76.
PMID: 17384084
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Lee JH et al. (2006-03-01), Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci., 2006-047(3):864-72.
PMID: 16505018
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Groschl M et al. (2005-04-01), Salivary leptin induces increased expression of growth factors in oral keratinocytes. J. Mol. Endocrinol., 2005-034(2):353-66.
PMID: 15821102

There are no citations that match your criteria.

Showing 1 - 6 of 6 citations Showing All

Customer Reviews for Human KGF/FGF‑7 Antibody (2)

4 out of 5

2 customer ratings

5 stars

4 stars

100%

3 stars

2 stars

1 star

Showing 1 - 2 of 2 reviews Showing All