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Human GloLIVE TRA-1-60(R) NorthernLights™ NL557-conjugated Antibody

50X Concentration, Azide Free

Key Product Details

Detection of TRA-1-60(R) in Live BG01V Human Embryonic Stem Cells.
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NLLC4770R

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, TRA-1-60(R) expression can be verified in live cells prior to colony selection or experimentation using this simple procedure:

  • Add the TRA-1-60(R) antibody directly to the media of cultured cells
  • Wash the cells after a 30 minute incubation
  • Select for and expand TRA-1-60(R)-positive colonies
 
 

Precautions

Use aseptic technique and sterile culturing conditions to prevent contamination.

Reagents Provided

GloLIVE NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody. (Catalog # NLLC4770R):

Other Supplies Required

Reagents

  • Stem cell culture media

Materials

  • Human or mouse pluripotent stem cells
  • Pipettes and pipette tips
  • Serological pipette

Equipment

  • 37 °C and 5% CO2 incubator
  • Fluorescence microscope
 

Live Cell Immunocytochemistry Procedure Overview

To ensure sterility of cultures, all steps should be performed under sterile conditions.


Add primary antibody in appropriate culture media to cells.
 
  1. Add fluorochrome-conjugated primary antibody in appropriate culture media to cells.
  2. Incubate for 30 minutes.

Replace with fresh culture media
 
  1. Replace with fresh culture media.

Visualize using a fluorescence microscope
 
  1. Visualize using a fluorescence microscope.

Note: Culture with GloLIVE antibodies does not appear to affect cell proliferation or stemness as assayed by proliferation curves for 3 days post-antibody incubation and expression levels of TRA-1-60(R) and Oct-3/4.

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  • Human GloLIVE TRA-1-60(R) NorthernLights™ NL557-conjugated Antibody
    Name: Anonymous
    Verified Customer | Posted 07/22/2021
    Human GloLIVE TRA-1-60(R) NorthernLights™ NL557-conjugated Antibody Serum NLLC4770R
  • Human GloLIVE TRA-1-60(R) NorthernLights™ NL557-conjugated Antibody
    Name: Anonymous
    Verified Customer | Posted 07/02/2021
    Human GloLIVE TRA-1-60(R) NorthernLights™ NL557-conjugated Antibody Serum NLLC4770R

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FAQs

  • What is the recommended starting confluence to observe staining?

    The optimal confluence would depend on the purpose of the staining. If the plan is to use all the cells in the flask after they have proliferated, then one can wait for the cells to become confluent or almost confluent (80-90% confluent) and then check the staining. If the goal is to to check on the cells mid-way through their growth and there are sufficient cells in the flask, one could check the staining then as well. The staining is not dependent on how many cells are in the flask, as long as there are sufficient cells.

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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