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Human GFAP Antibody
Catalog # MAB2594 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 273807
Product Summary for Human GFAP Antibody
Immunogen
E. coli-derived recombinant human GFAP
Leu292-Met432
Accession # P14136
Leu292-Met432
Accession # P14136
Specificity
Detects human GFAP in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GFAP Antibody
Detection of Human GFAP by Western Blot.
Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GFAP in Rat Cortical Stem Cells.
GFAP was detected in immersion fixed differentiated rat cortical stem cells using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Human GFAP by Simple WesternTM.
Simple Western lane view shows lysates of human brain (cerebellum) tissue and human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51-52 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human GFAP Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed differentiated rat cortical stem cells differentiated for 7 days
Sample: Immersion fixed differentiated rat cortical stem cells differentiated for 7 days
Knockout Validated
GFAP was detected in immersion fixed U937 human histiocytic lymphoma cell line but is not detected in GFAP knockout (KO) U937 Human cell line
Simple Western
50 µg/mL
Sample: human brain (cerebellum) tissue and human brain (motor cortex) tissue
Sample: human brain (cerebellum) tissue and human brain (motor cortex) tissue
Western Blot
1 µg/mL
Sample: Human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue
Sample: Human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.7 using MAB2594 in the following applications:
Published Applications
Read 6 publications using MAB2594 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFAP
Product Documents for Human GFAP Antibody
Product Specific Notices for Human GFAP Antibody
For research use only
Citations for Human GFAP Antibody (6)
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Citations are publications that use Bio-Techne products. Selected citations for Human GFAP Antibody include:
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Species: Human
Sample Types: Whole Tissue
Applications: IHCCruz-Orengo L et al. (2014-05-08), Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility. J Clin Invest, 2014-0124(6):2571-84.
PMID: 24812668 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCZeng et al. (2013-10-24), MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain. Gene Ther, 2013-121(1):37-43.
PMID: 24152581 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCMangiola et al. (2013-03-05), Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study. PLoS ONE, 2013-08(3):e57145.
PMID: 23472076 -
Species: Human
Sample Types: Whole Cells
Applications: ICCWalter D et al. (2011-05-13), CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres. PLoS ONE, 2011-06(5):e19506.
PMID: 21602936 -
Species: Human
Sample Types: Whole Cells
Applications: ICCThon N et al. (2008-08-07), Presence of pluripotent CD133+ cells correlates with malignancy of gliomas. Mol. Cell. Neurosci., 2008-043(1):51-9.
PMID: 18761091 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCPonomarev ED et al. (2005-04-26), Development of a culture system that supports adult microglial cell proliferation and maintenance in the resting state. J. Immunol. Methods, 2005-0300(1):32-46.
PMID: 15893321
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Customer Reviews for Human GFAP Antibody (3)
4.7 out of 5
3 customer
ratings
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Application: Western BlotSample Tested: AstrocytesSpecies: HumanVerified Customer | Posted 10/10/2021
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Application: ImmunocytochemistrySample Tested: Pig TM cellsSpecies: PigVerified Customer | Posted 06/07/2017
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Application: ImmunofluorescenceSample Tested: See PMID 22985741Species: HumanVerified Customer | Posted 02/19/2015
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