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Human GFAP Antibody

Catalog # MAB2594 | R&D Systems, Inc. a Bio-Techne Brand
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MAB2594
MAB2594-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Knockout Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 273807

Product Summary for Human GFAP Antibody

Immunogen

E. coli-derived recombinant human GFAP
Leu292-Met432
Accession # P14136

Specificity

Detects human GFAP in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GFAP Antibody

Detection of Human GFAP antibody by Western Blot.

Detection of Human GFAP by Western Blot.

Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GFAP antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

GFAP in Rat Cortical Stem Cells.

GFAP was detected in immersion fixed differentiated rat cortical stem cells using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Human GFAP antibody by Simple WesternTM.

Detection of Human GFAP by Simple WesternTM.

Simple Western lane view shows lysates of human brain (cerebellum) tissue and human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51-52 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human GFAP Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed differentiated rat cortical stem cells differentiated for 7 days

Knockout Validated

GFAP was detected in immersion fixed U937 human histiocytic lymphoma cell line but is not detected in GFAP knockout (KO) U937 Human cell line

Simple Western

50 µg/mL
Sample: human brain (cerebellum) tissue and human brain (motor cortex) tissue

Western Blot

1 µg/mL
Sample: Human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using MAB2594 in the following applications:

Published Applications

Read 6 publications using MAB2594 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFAP

Glial Fibrillary Acidic Protein (GFAP) is the predominant component of astrocyte intermediate filaments in the central nervous system. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous systems.

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

GFAP, ALXDRD, FLJ45472, GFAP astrocytes, glial fibrillary acidic protein

Entrez Gene IDs

2670 (Human); 14580 (Mouse); 24387 (Rat)

Gene Symbol

GFAP

UniProt

Product Documents for Human GFAP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human GFAP Antibody

For research use only

Citations for Human GFAP Antibody (6)

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Customer Reviews for Human GFAP Antibody (3)

4.7 out of 5
3 customer ratings
5 stars
67%
4 stars
33%
3 stars
0%
2 stars
0%
1 star
0%

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  • Human GFAP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Astrocytes
    Species: Human
    Verified Customer | Posted 10/10/2021
    Human GFAP Antibody MAB2594
  • Human GFAP Antibody
    Name: L P
    Application: Immunocytochemistry
    Sample Tested: Pig TM cells
    Species: Pig
    Verified Customer | Posted 06/07/2017
    Human GFAP Antibody MAB2594
  • Name: cynthia morimoto
    Application: Immunofluorescence
    Sample Tested: See PMID 22985741
    Species: Human
    Verified Customer | Posted 02/19/2015

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Reconstitution Calculator

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