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Human FGF-19 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB969

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MAB969-100
MAB969-SP
MAB969-500

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 117601

Product Summary for Human FGF-19 Antibody

Immunogen

E. coli-derived recombinant human FGF-19
Phe27-Lys216
Accession # O95750

Specificity

Detects human FGF-19 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF‑4, -5, -6, -7, -9, -10, -17, -18, recombinant mouse FGF-8b, -8c, or -15 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FGF-19 Antibody

FGF-19 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

FGF‑19 in Human Prostate Cancer Tissue.

FGF-19 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human FGF-19 Monoclonal Antibody (Catalog # MAB969) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human FGF-19 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample:  Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Western Blot

1 µg/mL
Sample: Recombinant Human FGF-19 (Catalog # 969-FG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB969 in the following applications:

Published Applications

Read 4 publications using MAB969 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-19

Fibroblast growth factor 19 (FGF-19) belongs to the large FGF family which has at least 23 members (1, 2). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (3, 4). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (4).

Human FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites (1, 2). Among FGF family members, human FGF-19 is most closely related to chicken FGF-19 and murine FGF-15, sharing approximately 61% and 51% aa sequence identity, respectively (1, 2, 5). Neither the human orthologue of mouse FGF-15, nor the mouse counterpart of human FGF-19 has been identified. With the exception of adult gall bladder epithelium, FGF-19 expression is restricted to fetal tissues (1, 2). Unlike most FGFs which bind to and activate more than one FGF receptor, FGF-19 is a specific ligand for FGF R4 (2). Similarly, another FGF family member, FGF-7 (KGF), only activates KGF R, the IIIb isoform of FGF R2 (4). During chick embryogenesis, FGF-19 has been shown to act synergistically with Wnt-8c to initiate inner ear development (5).

References

  1. Nishimura, T. et al. (1999) Biocheim. Biophys. Acta 1444:148.
  2. Xie, M. et al. (1999) Cytokine 11:729.
  3. Goldfarb, M. (1996) Cytokine & Growth Factor Reviews 7:311.
  4. Green, P. et al. (1996) BioEssays 18:639.
  5. Ladher, R.K. et al. (2000) Science 290:1965.

Long Name

Fibroblast Growth Factor 19

Alternate Names

FGF19

Entrez Gene IDs

9965 (Human)

Gene Symbol

FGF19

UniProt

Additional FGF-19 Products

Product Documents for Human FGF-19 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FGF-19 Antibody

For research use only

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