Human CXCL13/BLC/BCA‑1 PE‑conjugated Antibody

Catalog # IC801P | R&D Systems, Inc. a Bio-Techne Brand
Discontinued Product
IC801P has been discontinued. View all CXCL13/BLC/BCA-1 products.

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 53610

Product Summary for Human CXCL13/BLC/BCA‑1 PE‑conjugated Antibody

Immunogen

E. coli-derived recombinant human CXCL13/BLC/BCA-1
Val23-Arg94
Accession # O43927

Specificity

Detects human CXCL13/BLC/BCA-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL1, rhCXCL2, rhCXCL3, rhCXCL8/IL-8, rhCXCL10, rhCXCL9, rhCXCL12/SDF-1 alpha, or rhCXCL12/SDF-1 beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CXCL13/BLC/BCA‑1 PE‑conjugated Antibody

Detection of CXCL13/BLC/BCA-1 antibody in Human Immature Dendritic Cells antibody by Flow Cytometry.

Detection of CXCL13/BLC/BCA‑1 in Human Immature Dendritic Cells by Flow Cytometry.

Human immature dendritic cells (CD14+ PBMC cultured with 20 ng/mL rhIL-4 and 50 ng/mL rhGM-CSF for 7 days) were stained with Mouse Anti-Human CXCL13/BLC/BCA-1 PE-conjugated Monoclonal Antibody (Catalog # IC801P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human CXCL13/BLC/BCA‑1 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human immature dendritic cells (CD14+ PBMC cultured with 20 ng/mL rhIL-4 and 50 ng/mL rhGM-CSF for 7 days) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using IC801P in the following applications:

Published Applications

Read 1 publication using IC801P in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References

  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.

Alternate Names

ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Gene Symbol

CXCL13

UniProt

O43927

Product Documents for Human CXCL13/BLC/BCA‑1 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCL13/BLC/BCA‑1 PE‑conjugated Antibody

For research use only

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  • Human CXCL13/BLC/BCA-1 PE-conjugated Antibody
    Name: Anonymous
    Application: Using intracellular flow protocol
    Sample Tested: 22RV1
    Species: Human
    Verified Customer | Posted 06/08/2022
    Human CXCL13/BLC/BCA‑1 PE‑conjugated Antibody IC801P

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