Human CXCL13/BLC/BCA-1 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC801P

Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Val23-Arg94
Accession # O43927
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human CXCL13/BLC/BCA-1 PE-conjugated Antibody
Detection of CXCL13/BLC/BCA‑1 in Human Immature Dendritic Cells by Flow Cytometry.
Human immature dendritic cells (CD14+ PBMC cultured with 20 ng/mL rhIL-4 and 50 ng/mL rhGM-CSF for 7 days) were stained with Mouse Anti-Human CXCL13/BLC/BCA-1 PE-conjugated Monoclonal Antibody (Catalog # IC801P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human CXCL13/BLC/BCA-1 PE-conjugated Antibody
Intracellular Staining by Flow Cytometry
Sample: Human immature dendritic cells (CD14+ PBMC cultured with 20 ng/mL rhIL-4 and 50 ng/mL rhGM-CSF for 7 days) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Reviewed Applications
Read 1 review rated 4 using IC801P in the following applications:
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCL13/BLC/BCA-1
CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.
References
- Gunn, M.D. et al. (1998) Nature, 391:799.
- Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
- Forster, R. et al. (1996) Cell 87:1037.
Alternate Names
Gene Symbol
UniProt
Additional CXCL13/BLC/BCA-1 Products
Product Documents for Human CXCL13/BLC/BCA-1 PE-conjugated Antibody
Product Specific Notices for Human CXCL13/BLC/BCA-1 PE-conjugated Antibody
For research use only