Human CD45RO PE-conjugated Antibody

Name: Human CD45RO PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB10642P

R&D Systems, part of Bio-Techne | Catalog # FAB10642P

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Discontinued Product

FAB10642P has been discontinued. View all CD45RO products.

Key Product Details

Species Reactivity:	Human
Immunogen:	Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG_2A Clone # UCHL1
Specificity:	Detects human CD45RO in direct ELISAs.

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Technical Data
Citations / Reviews

Applications for Human CD45RO PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	Human PBMC

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD45RO PE-conjugated Antibody

	Flow Cytometry Detection of CD45RO in Human PBMC Lymphocytes by Flow Cytometry. Human PBMC were stained with (A) Mouse Anti-Human CD45RO PE-conjugated Monoclonal Antibody (Catalog # FAB10642P) or (B) Mouse IgG2A Isotype Control Antibody (IC003P) and Mouse Anti-Human CD45RA APC-conjugated Monoclonal Antibody. Staining was performed using our Staining Membrane-associated Proteins protocol.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: CD45RO

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). Multiple splicing isoforms of exon 4 (A), 5 (B), and 6 (C) are expressed according to cell type, developmental stage and antigenic exposure (1-5). The longest form, CD45RABC (called B220 in mouse) is expressed on B lymphocytes, The shortest form, CD45R0, lacking exons 4, 5 and 6 which encode aa 34‑194, is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). Human CD45 has a 40% and 41% sequence identity with mouse and rat respectively. The CD45R0 cDNA encodes 1145 amino acids (aa), including a 25 aa signal sequence, a 391 aa extracellular domain, 21 aa transmembrane sequence, and a 708 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily O‑glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

Item	Value
References	Anderson, J.N. et al. (2004) FASEB J. 18:8. Streuli, M. et al. (1987) J. Exp. Med. 166:1548. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608. Ralph, S.J. et al. (1987) EMBO J. 6:1251. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146. McNiell, L. et al. (2007) Immunity 27:425. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911. Zhu, J.W. et al. (2008) Immunity 28:183. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
Long Name	CD45R, O isoform
Entrez Gene IDs	5788 (Human); 19264 (Mouse)
Alternate Names	B220, CD45 antigen, CD45R, CD45RO, EC 3.1.3.48, L-CA, LY5, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C

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Human CD45RO PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB10642P

Key Product Details

Applications for Human CD45RO PE-conjugated Antibody

Scientific Data Examples for Human CD45RO PE-conjugated Antibody

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: CD45RO

Customer Reviews for Human CD45RO PE-conjugated Antibody

Product Documents for Human CD45RO PE-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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