Human CD3 epsilon Antibody
Catalog # MAB10670 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
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Product Summary for Human CD3 epsilon Antibody
Immunogen
Gln22-Asp126
Accession # P07766
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human CD3 epsilon Antibody
Detection of Human CD3 epsilon by Western Blot.
Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB10670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD3 epsilon at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.CD3 epsilon in Human Thymus.
CD3 epsilon was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB10670) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Detection of Human CD3 epsilon by Simple WesternTM.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for CD3 epsilon at approximately 30 kDa (as indicated) using 25 µg/mL of Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB10670) . This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.Applications for Human CD3 epsilon Antibody
Immunohistochemistry
Sample: Immersion fixed paraffinembedded sections of human thymus
Simple Western
Sample: Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line
Western Blot
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD3 epsilon
References
- Gold, D.P. et al. (1986) Nature 321:431.
- Barber, E.K. et al. (1989) Proc. Nat. Acad. Sci. U.S.A. 86:3277.
- Manolios, N. et al. (1991) EMBO J 10:1643.
- Dietrich, J. et al. (1999) Eur J Immunol 29:1719.
- Pan, Q. et al. (2006) Mol Immunol 43:1741.
- Soudais, C. et al. (1993) Nat. Genet. 3:77.
Alternate Names
Gene Symbol
UniProt
Product Documents for Human CD3 epsilon Antibody
Product Specific Notices for Human CD3 epsilon Antibody
For research use only
FAQs for Human CD3 epsilon Antibody
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Q: What is the optimal working concentration of the antibody?
A: Novus' product datasheets provide a recommended concentration for each validated application that is a starting point for titration. The concentration required will vary from lab to lab due to other variables and thus titration is strongly recommended for optimizing results.
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.