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Human CD3 epsilon Antibody

Catalog # MAB10670 | R&D Systems, Inc. a Bio-Techne Brand
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MAB10670-100
MAB10670-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1033713

Product Summary for Human CD3 epsilon Antibody

Immunogen

Human embryonic kidney cell HEK293-derived human CD3 epsilon
Gln22-Asp126
Accession # P07766

Specificity

Detects human CD3 epsilon in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD3 epsilon Antibody

Detection of Human CD3 epsilon by Western Blot.

Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB10670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD3 epsilon at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

CD3 epsilon in Human Thymus.

CD3 epsilon was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB10670) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human CD3 epsilon by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for CD3 epsilon at approximately 30 kDa (as indicated) using 25 µg/mL of Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB10670) . This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Applications for Human CD3 epsilon Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffinembedded sections of human thymus

Simple Western

25 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line

Western Blot

2 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3 epsilon

CD3 epsilon (T-Cell Surface Glycoprotein CD3 Epsilon) is a type I transmembrane protein that belongs to the Ig (Immunoglobulin) superfamily. Human CD3 epsilon consists of 104 amino acid (aa) extracellular domain, 26 aa transmembrane domain and 55 aa cytoplasmic domain (1). With the extracellular domain, human CD3 epsilon shares 44.2% and 49% homology with mouse and rat CD3 epsilon respectively. There are four CD3 proteins: CD3 delta, CD3 epsilon, CD3 gamma, and CD3 zeta. All CD3 proteins contain ITAM (Immunoreceptor Tyrosine-based Activation Motifs) in the cytoplasmic tail, which becomes phosphorylated by Src family protein tyrosine kinases LCK and FYN upon TCR engagement (2). CD3 proteins form heterodimers of CD3 delta/CD3 epsilon and CD3 gamma/CD3 epsilon, which bind to TCR and form trimeric TCR alpha/CD3 epsilon/CD3 gamma and TCR beta/CD3 gamma/CD3 epsilon. The resulting heterohexamer further associates with CD3 zeta homodimer and forms TCR/CD3 signaling complex (3, 4). Similar complex is also formed with TCR gamma and TCR delta (5). CD3 epsilon plays essential role in adaptive immune response. Mutations in CD3 epsilon lead to SCID (severe combined immunodeficiency) (6).

References

  1. Gold, D.P. et al. (1986) Nature 321:431.
  2. Barber, E.K. et al. (1989) Proc. Nat. Acad. Sci. U.S.A. 86:3277.
  3. Manolios, N. et al. (1991) EMBO J 10:1643.
  4. Dietrich, J. et al. (1999) Eur J Immunol 29:1719.
  5. Pan, Q. et al. (2006) Mol Immunol 43:1741.
  6. Soudais, C. et al. (1993) Nat. Genet. 3:77.

Alternate Names

CD3e

Entrez Gene IDs

102133065 (Cynomolgus Monkey); 916 (Human); 12501 (Mouse)

Gene Symbol

CD3E

UniProt

Product Documents for Human CD3 epsilon Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD3 epsilon Antibody

For research use only

FAQs for Human CD3 epsilon Antibody

Showing  1 - 1 of 1 FAQ Showing All
  • Q: What is the optimal working concentration of the antibody?

    A: Novus' product datasheets provide a recommended concentration for each validated application that is a starting point for titration. The concentration required will vary from lab to lab due to other variables and thus titration is strongly recommended for optimizing results.

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